Microfluidics separation method and system thereof

A microfluidic system for separating an analyte from a sample fluid comprising:

• a) a series of fluidic channels comprising at least one first region and at least one second region;
• b) a sample inlet portion connected to a first end of said series of fluidic channels, wherein said sample inlet portion is configured to receive said sample fluid comprising said analyte;
• c) a buffer inlet portion connected to said first end of said series of fluidic channels, wherein said buffer inlet portion is configured to receive a buffer fluid;
• d) a recovery outlet portion connected to a second end of said series of fluidic channels;
• and
• e) a waste outlet portion connected to said second end of said series of fluidic channels,
• wherein said first region comprises a plurality of L-nodes, which connects to each other in series,
• wherein said second region comprises a plurality of R-nodes, which connects to each other in series,
• wherein said first region is configured to trigger at least about one lamination process cycle for both said sample fluid and said buffer fluid and said second region is configured to trigger at least about one reverse lamination process cycle for both said sample fluid and said buffer fluid, whereby said lamination process cycle and said reverse lamination process cycle causes said analyte to diffuse to said buffer fluid from said sample fluid, and
• wherein said recovery outlet portion is configured to transfer said analyte and said buffer fluid from said microfluidic system for collection.