Medicinal Composition of Amaranth Extract Having Enriched Nitrate Content and a Method of Preparing the same

A method of preparing a powdered extract of fresh Amaranth, the method comprising:

• crushing fresh leaves and stem of Amaranth to obtain a crushed material,
• extracting the crushed material with methanol to obtain a supernatant and residue,
• filtering the supernatant to obtain a filtrate;
• concentrating the filtrate to obtained a concentrated filtrate, and,
• drying the concentrated filtrate to obtain a powdered extract of fresh Amaranth.

A method of preparing a powdered extract of fresh Amaranth, the method comprising:

• crushing fresh leaves and stem of Amaranth to obtain a crushed material,
• extracting the crushed material with water to obtain a supernatant and a residue, and,
• concentrating the supernatant to obtain a concentrated water extract,
• drying the concentrated water extract to obtain a powdered extract of fresh Amaranth.

A method of preparing a powdered extract of dried Amaranth, the method comprising:

• powdering dried leaves and stem of Amaranth to obtain a powdered material,
• extracting the powdered material with methanol to obtain a supernatant,
• filtering the supernatant to obtain a filtrate,
• concentrating the filtrate to obtain a concentrated methanol filtrate, and,
• drying the concentrated methanol filtrate to obtain a powdered extract of dried Amaranth.

A method of preparing a powdered extract of dried Amaranth, the method comprising:

• powdering dried leaves and stem of Amaranth to obtain a powdered material,
• extracting the powdered material with water to obtain a supernatant and a residue,
• concentrating the supernatant to obtain a concentrated water extract, and,
• drying the concentrated water extract to obtain a powdered extract of dried Amaranth.

A method preparing an extract of Amaranth, the method comprising:

• crushing fresh leaves and stem of Amaranth to obtain a slurry,
• treating the slurry with pectinase to obtain a pectinase treated material,
• heating the pectinase treated material to obtain a pectinase-deactivated material,
• extracting the pectinase-deactivated material with water to obtain a supernatant and a residue,
• concentrating the supernatant to obtain a concentrated supernatant,
• drying the concentrated supernatant to obtain a powdered extract of fresh Amaranth.

A method of preparing an extract of Amaranth, the method comprising:

• crushing fresh leaves and stem of Amaranth to obtain a slurry,
• treating the slurry with pectinase to obtain a pectinase treated material,
• heating the pectinase treated material to obtain a pectinase-deactivated material,
• extracting the pectinase-deactivated material with water to obtain a supernatant and a residue,
• concentrating the supernatant to obtain a concentrated water extract,
• cooling the concentrated water extract at 10° C. for 48 hours then filtering to obtain crystals of oxalic acid and/or oxalate and a second supernatant;
• cooling the second supernatant at 10° C. for 24 hours to obtain a third supernatant and a second oxalic acid and/or oxalate crystals;
• filtering the third supernatant to obtain a filtrate,
• concentrating the filtrate to obtain a concentrated filtrate, and,
• drying the concentrated filtrate to obtain a powdered extract, wherein the powdered extract comprises less than 0.1% total oxalic acid, wherein total oxalic acid comprises free oxalic acid and oxalates.

A method of preparing an extract of Amaranth, the method comprising:

• crushing fresh leaves and stem of Amaranth to obtain a slurry,
• treating the slurry with pectinase to obtain a pectinase treated material,
• heating the pectinase treated material to obtain a pectinase-deactivated material,
• extracting the pectinase-deactivated material with water to obtain a supernatant and a residue,
• concentrating the supernatant to obtain a concentrated water extract,
• cooling the concentrated water extract at 10° C. for 48 hours then filtering to obtain crystals of oxalic acid and/or oxalate and a second supernatant;
• cooling the second supernatant at 10° C. for 24 hours to obtain a third supernatant and a second oxalic acid and/or oxalate crystals;
• filtering the third supernatant to obtain a filtrate,
• concentrating the filtrate to obtain a concentrated filtrate, and,
• drying the concentrated filtrate to obtain a powdered extract,
• treating the powdered extract with hexane to obtain a mixture,
• filtering the mixture to obtain a filtrate,
• concentrating the filtrate to obtain a concentrated hexane extract,
• drying the concentrated hexane extract to obtain a powdered hexane extract.

A method of preparing an extract of Amaranth, the method comprising:

• crushing fresh leaves and stem of Amaranth to obtain a slurry,
• treating the slurry with pectinase to obtain a pectinase treated material,
• heating the pectinase treated material to obtain a pectinase-deactivated material,
• extracting the pectinase-deactivated material with water to obtain a supernatant and a residue,
• concentrating the supernatant to obtain a concentrated water extract,
• cooling the concentrated water extract at 10° C. for 48 hours then filtering to obtain crystals of oxalic acid and/or oxalates and a second supernatant;
• cooling the second supernatant at 10° C. for 24 hours to obtain a third supernatant and a second oxalic acid and/or oxalate crystals;
• filtering the third supernatant to obtain a filtrate,
• concentrating the filtrate to obtain a concentrated filtrate, and,
• drying the concentrated filtrate to obtain a powdered extract,
• treating the powdered extract with chloroform to obtain a mixture,
• filtering the mixture to obtain a filtrate,
• concentrating the filtrate to obtain a concentrated chloroform extract,
• drying the concentrated chloroform extract to obtain a powdered chloroform extract.

A method of preparing an extract of Amaranth, the method comprising:

• crushing fresh leaves and stem of Amaranth to obtain a slurry,
• treating the slurry with pectinase to obtain a pectinase treated material,
• heating the pectinase treated material to obtain a pectinase-deactivated material,
• extracting the pectinase-deactivated material with water to obtain a supernatant and a residue,
• concentrating the supernatant to obtain a concentrated water extract,
• cooling the concentrated water extract at 10° C. for 48 hours then filtering to obtain crystals of oxalic acid and/or oxalate and a second supernatant;
• cooling the second supernatant at 10° C. for 24 hours to obtain a third supernatant and a second oxalic acid and/or oxalate crystals;
• filtering the third supernatant to obtain a filtrate,
• concentrating the filtrate to obtain a concentrated filtrate, and,
• drying the concentrated filtrate to obtain a powdered extract,
• treating the powdered extract with ethylene dichloride to obtain a mixture,
• filtering the mixture to obtain a second filtrate,
• concentrating the second filtrate to obtain a concentrated ethylene dichloride extract,
• drying the concentrated ethylene dichloride extract to obtain a powdered ethylene dichloride extract.

A method of preparing an extract of Amaranth additionally with Amla extract, the method comprising:

• crushing fresh leaves and stem of Amaranth to obtain a slurry,
• treating the slurry with pectinase to obtain a pectinase treated material,
• heating the pectinase treated material to obtain a pectinase-deactivated material,
• extracting the pectinase-deactivated material with water to obtain a supernatant and a residue,
• concentrating the supernatant to obtain a concentrated water extract,
• cooling the concentrated water extract at 10° C. for 48 hours then filtering to obtain crystals of oxalic acid and/or oxalate and a second supernatant;
• cooling the second supernatant at 10° C. for 24 hours to obtain a third supernatant and a second oxalic acid and/or oxalate crystals;
• filtering the third supernatant to obtain a filtrate,
• concentrating the filtrate to obtain a concentrated filtrate, and,
• drying the concentrated filtrate to obtain a powdered extract,
• treating the powdered extract with hexane to obtain a mixture,
• filtering the mixture to obtain a second filtrate,
• concentrating the second filtrate to obtain a concentrated hexane extract,
• drying the concentrated hexane extract to obtain a powdered hexane extract,
• dissolving the powdered hexane extract in methanol to obtain a solution of hexane extract of Amaranth,
• adding a powder of methanol extract of Amla to the solution of hexane extract of Amaranth to obtain a second mixture,
• evaporating the second mixture to obtain an concentrate,
• drying the concentrate to obtain a powder of Amaranth and Amla extract,
• wherein the powdered methanol extract of Amla extract is prepared by a process comprising:
• pulping fresh Amla fruit to obtain a pulp,
• extracting the pulp with methanol to obtain a filtrate,
• concentrating the filtrate to obtain a concentrated methanol extract of Amla,
• drying the concentrated methanol extract of Amla to obtain the powdered methanol extract of Amla.