Method and Composition for Hypothermic Storage of Placental Tissue
AssigneeNuTech Medical, Inc.
InventorsHoward P. Walthall, JR., Katie C. Mowry, Jeremy B. Vines
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Abstract
A tissue storage solution includes hypothermic storage compositions and methods. The hypothermic storage composition includes media containing Dulbecco's modified Eagle's medium (DMEM) and albumin. A method of hypothermically storing tissue includes storing such tissue in a storage medium including DMEM and albumin. A method for wound or defect treatment includes applying tissue, stored in a hypothermic storage medium containing DMEM and albumin, to the site of such wound or defect.
Claims29
A method for treating a wound comprising:
- hypothermically storing a placental membrane in a container including a solution including Dulbecco's modified Eagle's medium (DMEM) and human plasma albumin, wherein the human plasma albumin promotes cell viability and membrane integrity of the placental membrane,
- removing the placental membrane from the container, and
- applying the placental membrane to a wound.
A method of treating a wound comprising storing a placental membrane for a period of at least fourteen days, and thereafter, applying the placental membrane to a wound wherein the placental membrane exhibits a ratio of live cells to dead cells of at least 1:1 when applied to the wound.
A method a treating a wound comprising:
- storing amniotic tissue in a container for at least fourteen days, the container including Dulbecco's modified Eagle's medium (DMEM) and human plasma albumin, and thereafter,
- applying the amniotic tissue to the wound,
- wherein the amniotic tissue includes more live cells than dead cells when applied to the wound.
Dependencies are inferred from the claim text.