Kits and methods for processing stem cells from bone marrow or umbilical cord blood
AssigneeMingqi TANG
InventorMingqi TANG
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Abstract
Provided herein are kits for processing stem cells from bone marrow or umbilical cord blood, comprising: a) a precipitation reagent comprising an aqueous solution of 0.1-30% hydroxyethyl starch or 0.1-30% methyl cellulose, and 0.1-20% of cerebroprotein hydrolysate; and b) a separation reagent comprising an aqueous solution comprising Ficoll and diatrizoate and having a density of 1.0-1.2 g/ml, and methods of use. Further provided herein is a collection of stem cells obtained by the kits and methods disclosed herein comprising stem cells from bone marrow or umbilical cord blood, and uses thereof.
Claims28
A kit for processing stem cells from bone marrow or umbilical cord blood, comprising:
- a) a precipitation reagent comprising an aqueous solution of 0.1-30% hydroxyethyl starch or 0.1-30% methyl cellulose, and 0.1-20% of cerebroprotein hydrolysate;
- and
- b) a separation reagent comprising an aqueous solution comprising Ficoll and diatrizoate and having a density of 1.0-1.2 g/ml.
18-37. (canceled)
A method of treating a disease condition in a subject comprising administrating a collection of stem cells to said subject, wherein the collection of stem cells comprises stem cells from bone marrow or umbilical cord blood and is obtained by a method for separating stem cells using a kit for processing stem cells from bone marrow or umbilical cord blood comprising a precipitation reagent comprising an aqueous solution of 0.1-30% hydroxyethyl starch or 0.1-30% methyl cellulose, and 0.1-20% of cerebroprotein hydrolysate;
- and a separation reagent comprising an aqueous solution comprising Ficoll and diatrizoate and having a density of 1.0-1.2 g/ml, which method comprises:
- a) providing a bone marrow or umbilical cord blood sample;
- b) adding the precipitation reagent to the bone marrow or umbilical cord blood sample, mixing and letting the solution to settle, followed by centrifugation of the top solution to obtain a concentrated sample;
- and
- c) adding the concentrated sample from step b) on top of the separation reagent to obtain a collection of cells,
- wherein the collection of cells comprises stem cells.
Dependencies are inferred from the claim text.