Organoids comprising isolated renal cells and use thereof
AssigneeRegenmedtx, LLC
InventorsJoydeep Basu, Andrew Bruce, Rusty Kelley, Kelley Guthrie
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Abstract
Described herein are organoids comprising admixtures of selected bioactive primary renal cells and a bioactive cell population, e.g., an endothelial cell populations, e.g. HUVEC cells, and methods of treating a subject in need thereof with such organoids. Further, the isolated renal cells, which may include tubular and erythropoietin {EPO}-producing kidney cell populations, and/or the endothelial cell populations may be of autologous, syngeneic, allogeneic or xenogeneic origin, or any combination thereof. Further provided are methods of treating a subject in need with the organoids.
Claims52
A method of forming an organoid comprising a heterogeneous renal cell population and a bioactive cell population, said method comprising culturing the heterogenerous renal cell population and a bioactive cell population in a culture system selected from the group consisting of i) 2D culture;
- ii) 3D culture:
- COL(I) gel;
- iii) 3D culture:
- Matrigel;
- iv) 3D culture:
- spinners, followed by COL(I)/Matrigel;
- and v) 3D culture:
- COL(IV) gel.
An organoid comprising a heterogeneous renal cell population and a bioactive cell population.
An injectable formulation comprising at least one organoid and a liquid medium.
An injectable formulation comprising organoids and a temperature-sensitive cell-stabilizing biomaterial that maintains
- (i) a substantially solid state at about 8° C. or below, and
- (ii) a substantially liquid state at about ambient temperature or above.
A method of treating kidney disease in a subject in need comprising administering at least one organoid comprising a heterogeneous renal cell population and a bioactive cell population.
Use of an organoid in the preparation of a medicament for the treatment of kidney disease.
Dependencies are inferred from the claim text.