Method for producing a mixture of neutral oligosaccharides extracted from flaxseed
AssigneesUniversite De Reims Champagneardenne, Centre Valorisation Glucides Prod Nat, Centre National De La Recherche Scientifique, Vandeputte Oleochemicals, Universite De Rouen
InventorsFrancois MAQUART, Georges BELLON, Claire MARCHAL, Helene DUCATEL, Olivier DUPUIS, Luc PICTON, Didier LECERF, Renauld FORBICE
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Abstract
The method for producing a mixture of neutral oligosaccharides extracted from flaxseed includes oligosaccharides having high molar masses generated by fractionation by ultrafiltration at a cut-off of between 15,000 and 50,000 Da and between 5,000 and 15,000 Da. The mixture of oligosaccharides produced as a result of the implementation of the method, and to the applications of the mixture can be used for skin repair and skin aging control.
Claims19
A method for obtaining a composition of neutral oligosaccharides extracted from flaxseed, said oligosaccharides having high molecular weights, said method including the following steps:
- carrying out a hydrolysis at acidic pH on a solution of flax mucilage, said flax mucilage being obtained by extraction from flaxseed in a solvent;
- neutralizing said solution by adding a base in an adequate quantity so as to form a neutralized solution;
- carrying out a first ultrafiltration of said neutralized solution through a membrane with a porosity of 50,000 Da so as to obtain a first retentate and a first permeate;
- carrying out a second ultrafiltration of said first permeate through a membrane with a porosity of 15,000 Da so as to obtain a second retentate and a second permeate;
- carrying out a third ultrafiltration of said second permeate through a membrane with a porosity of 5,000 Da so as to obtain a third retentate and a third permeate;
- and
- mixing said second retentate and said third retentate in order to obtain a mixture of oligosaccharides, said second retentate being comprised of oligosaccharides having molar weights resulting from fractionation by said second ultrafiltration at a cutoff point between 15,000 and 50,000 Da, and said third retentate being comprised of oligosaccharides having molar weights resulting from fractionation by said third ultrafiltration at a cutoff point between 5,000 and 15,000 Da.
Dependencies are inferred from the claim text.