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US 2016/0100570 A1Published application
Ultra-high density cell banking methods
AssigneeGenzyme Corporation
InventorsXiaoxia Jin, Claudia Buser
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Abstract
Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.
Claims33
A method for producing an ultra-high density frozen cell bank directly from a population of cultured cells, the method comprising:
- a) culturing cells in a perfusion bioreactor to obtain an ultra-high density cell population with a concentration of at least about 1.0×10̂8 cells/mL, wherein the perfusion bioreactor is coupled to a cell retention system;
- and
- b) adding cryoprotectant to the ultra-high density cell population to produce an ultra-high density frozen cell bank, wherein the ultra-high density frozen cell bank has a concentration of at least about 1.0×10̂8 cells/mL, and
- wherein no additional concentrating step is performed between culturing the cells and adding cryoprotectant to the ultra-high density cell population.
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Dependencies are inferred from the claim text.