Method for detecting nucleosome adducts
AssigneeSingapore Volition Pte Limited
InventorsJacob Vincent MICALLEF, Mark Edward ECCLESTON, Marielle HERZOG
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Abstract
The invention relates to a method for detecting and measuring the presence of nucleosome-protein adducts and the use of such measurements for the detection and diagnosis of disease. The invention also relates to a method of identifying nucleosome adduct biomarkers for the detection and diagnosis of disease and to biomarkers identified by said method.
Claims15
-31. (canceled)
A method for detecting the presence of a nucleosome-protein adduct in a sample, wherein the nucleosome-protein adduct comprises a polycomb protein, a transcription factor, a DNA damage repair protein, a transcription enhancement factor, a transcription repression factor, or a structural chromatin protein, which comprises the steps of:
- (i) contacting the sample with a first binding agent which binds to nucleosomes or a component thereof;
- (ii) contacting the nucleosomes or sample with a second binding agent which binds to a protein adducted to the nucleosome;
- (iii) detecting or quantifying the binding of said second binding agent to the adducted protein in the sample;
- and
- (iv) using the presence or degree of such binding as a measure of the presence of nucleosome adducts in the sample.
A method for detecting the presence of a nucleosome-protein adduct in a sample, wherein the nucleosome-protein adduct comprises a polycomb protein, a transcription factor, a DNA damage repair protein, a transcription enhancement factor, a transcription repression factor or a structural chromatin protein, which comprises the steps of:
- (i) contacting the sample with a first binding agent which binds to a protein adducted to the nucleosome;
- (ii) contacting the nucleosomes or sample with a second binding agent which binds to nucleosomes or a component thereof;
- (iii) detecting or quantifying the binding of said second binding agent to nucleosomes or a component thereof;
- and
- (iv) using the presence or degree of such binding as a measure of the presence of nucleosome adducts in the sample.
A method for detecting the presence of a nucleosome-protein adduct in a sample, wherein the nucleosome-protein adduct comprises a polycomb protein, a transcription factor, a DNA damage repair protein, a transcription enhancement factor, a transcription repression factor or a structural chromatin protein, which comprises the steps of:
- (i) contacting the sample with a first binding agent which binds to nucleosomes, a component thereof or a protein adducted to a nucleosome;
- (ii) contacting the nucleosomes or sample with a second binding agent which binds to nucleosomes or a component thereof when the first binding agent binds to the adducted protein, or said second binding agent binds a protein adducted to the nucleosome when the first binding agent binds to nucleosomes or a component thereof;
- (iii) detecting or quantifying the binding of said second binding agent to nucleosomes, a component thereof or the adducted protein in the sample;
- and
- (iv) using the presence or degree of such binding as a measure of the presence of nucleosome adducts in the sample.
Dependencies are inferred from the claim text.