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US 2016/0097768 A1Published application
Polyvalent chimeric ospc vaccinogen and diagnostic antigen
AssigneeVirginia Commonwealth University
InventorsRichard T. MARCONI, Christopher EARNHART, Lee D. OLIVER
Life of this patent
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Abstract
Chimeric polyvalent recombinant proteins for use as vaccines and diagnostics for Lyme disease (e.g. in canines and humans) are provided. The chimeric proteins comprise epitopes of the loop 5 region and/or the alpha helix 5 region of outer surface protein C (OspC) types and/or OspE types. The OspC types may be associated with mammalian Borrelia infections.
Claims5
A method for ascertaining whether an individual has been exposed to or infected with Borrelia, or both, comprising the steps of
- obtaining a biological sample from said individual,
- exposing said biological sample to
- at least one recombinant chimeric protein comprising epitopes from loop 5 region or alpha helix 5 region, or both, of two or more outer surface protein C (OspC) types which are different from each other;
- and
- at least one outer surface protein E (OspE) recombinant protein that does not bind factor H (FH);
- and
- determining whether antibodies in said biological sample bind to said at least one chimeric protein and said at least one OspE recombinant protein that does not bind FH, wherein detection of antibody binding is indicative of prior exposure to or infection with Borrelia.
4 more elements
Dependencies are inferred from the claim text.