Analyte mass spectrometry quantitation using a universal reporter
AssigneeThermo Fisher Scientific Inc.
InventorsJoel Louette, John Charles Rogers, Scott M. Peterman, Bruno Domon, Elodie Duriez
Life of this patent
Estimating the term…
Abstract
Quantitation of analytes, including but not limited to peptides, polypeptides, and proteins, in mass spectrometry using a labeled peptide coupled to a reporter, and a universal reporter.
Claims12
1-19. (canceled)
A method of assessing sensitivity limits of MS equipment, the method comprising
- generating a dilution curve in a single injection into a liquid chromatograph/mass spectrometer by injecting into a first liquid chromatography (LC) column coupled to a mass spectroscopy detection system a single peptide composition, the single peptide composition comprising a composition comprising at least a first set of a plurality of peptide isotopologues, where each peptide isotopologue of the first set has the same amino acid sequence;
- each peptide isotopologue of the first set has a different mass;
- and each peptide isotopologue of the first set is present at a different concentration;
- analyzing each peptide in the co-eluted peptide composition by mass spectroscopy;
- generating from the single peptide composition injection into the LC column at least a first dilution curve from the analysis;
- and
- using results of the at least first dilution curve to assess sensitivity of the MS equipment.
A method of assessing sensitivity limits of MS equipment, the method comprising
- generating a dilution curve in a single injection into a liquid chromatograph/mass spectrometer by injecting into a first liquid chromatography (LC) column coupled to a mass spectroscopy detection system a single peptide composition, the single peptide composition comprising a composition comprising at least a first set of a plurality of peptide isotopologues, where each peptide isotopologue of the first set has the same amino acid sequence and same unit mass;
- each peptide isotopologue of the first set has a unique distribution of isotopes such that peptide fragment ions for the same subsequence(s) have a different mass;
- and each peptide isotopologue of the first set is present at a different concentration;
- analyzing each peptide in the co-eluted peptide composition by mass spectroscopy;
- generating from the single peptide composition injection into the LC column at least a first dilution curve from the analysis;
- and
- using results of the at least first dilution curve to assess sensitivity of the MS equipment.
A composition comprising at least a first set of a plurality of peptide isotopologues, where each peptide isotopologue of the first set is present at a different concentration and has the same amino acid sequence.
Dependencies are inferred from the claim text.