Method for Detection of Analytes
AssigneesAlveo Technologies, Inc., The Regents of the University of California
InventorsSumita Pennathur, Peter Joseph Crisalli, Ronald Phillip Chiarello
Life of this patent
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Abstract
Embodiments provide analyte detection methods, techniques and processes for detecting the presence of one or more analytes in one or more samples. In a detection method, a sample and a sensor compound is introduced into a channel. A first potential difference is applied across the length of the channel in a first direction, and a first electrical property value is detected. Subsequently, a second potential difference is applied across the length of the channel in a second opposite direction, and a second electrical property value is detected. Presence or absence of an analyte in the channel is determined based on a comparison between the first and second electrical property values.
Claims28
A method for detecting the presence or absence of an analyte in a sample, the method comprising:
- introducing a sample into a channel, the channel having a length and a width, the length substantially greater than the width;
- measuring an electrical property value of an electrical property along at least a portion of the length of the channel after the sample is introduced into the channel;
- accessing a reference electrical property value, the reference electrical property value associated with the electrical property of the channel along at least a portion of the length of the channel prior to introduction of the sample into the channel;
- comparing the measured electrical property value and the reference electrical property value;
- and
- determining whether an analyte is present in the channel based on the comparison between the measured electrical property value and the reference electrical property value.
A method for detecting the presence or absence of an analyte in a sample, the method comprising:
- introducing a sensor compound into a channel, the channel having a length and a width, the length being substantially greater than the width;
- measuring one or more electrical properties of the channel along at least a portion of the length of the channel;
- determining a reference channel electrical property value based on the one or more electrical properties of the channel measured during the previous measuring step;
- introducing a sample into the channel;
- measuring the one or more electrical properties of the channel along at least the portion of the length of the channel after the sample and the sensor compound are introduced into the channel;
- determining an electrical property value based on the one or more electrical properties measured after the sensor compound and the sample are introduced into the channel;
- determining any differences between the reference channel electrical property value and the electrical property value;
- and
- determining whether an analyte is present in the channel based on the differences, if any, between the reference channel electrical property value and the electrical property value.
A method for detecting the presence or absence of an analyte in a sample, the method comprising:
- introducing a sample into a channel, the channel having a length and a width, the length being substantially greater than the width;
- measuring one or more electrical properties of the channel along at least a portion of the length of the channel;
- determining a reference channel electrical property value based on the one or more electrical properties of the channel measured during the previous measuring step;
- introducing a sensor compound into the channel;
- measuring the one or more electrical properties of the channel along at least the portion of the length of the channel after the sample and the sensor compound are introduced into the channel;
- determining an electrical property value based on the one or more electrical properties measured after the sensor compound and the sample are introduced into the channel;
- determining any differences between the reference channel electrical property value and the electrical property value;
- and
- determining whether an analyte is present in the channel based on the differences, if any, between the reference channel electrical property value and the electrical property value.
A method for detecting the presence or absence of an analyte in a sample, the method comprising:
- coating at least a portion of an inner surface of a channel with a sensor compound, the channel having a length and a width, the length substantially greater than the width;
- measuring one or more electrical properties of the channel along at least a portion of the length of the channel after the channel is coated with the sensor compound;
- determining a reference channel electrical property value based on the one or more electrical properties of the channel measured during the previous measuring step;
- and
- storing the reference channel electrical property value for use in determining whether or not an analyte is present in a sample introduced in the channel.
A method for detecting the presence or absence of an analyte in a sample, the method comprising:
- introducing a sample and a sensor compound into a channel, the channel having a length and a width, the length substantially greater than the width;
- applying a first potential difference across the length of the channel in a first direction along the length of the channel;
- measuring a first electrical property value of an electrical property along at least a portion of the length of the channel while the first potential difference is applied;
- applying a second potential difference across the length of the channel in a second direction along the length of the channel, the second direction opposite to the first direction;
- measuring a second electrical property value of the electrical property along at least the portion of the length of the channel while the second potential difference is applied;
- comparing the first and second electrical property values;
- and
- determining whether an analyte is present in the channel based on the comparison between the first and second electrical property values.
Dependencies are inferred from the claim text.