Engineered leather and methods of manufacture thereof
OwnerGabor FORGACS, Francoise Suzanne MARGA +1inventors
InventorsGabor FORGACS, Francoise Suzanne MARGA, Karoly Robert JAKAB
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Abstract
Engineered animal skin, hide, and leather comprising a plurality of layers of collagen formed by cultured animal collagen-producing (e.g., skin) cells. Layers may be formed by elongate multicellular bodies comprising a plurality of cultured animal cells that are adhered and/or cohered to one another; wherein the elongate multicellular bodies are arranged to form a substantially planar layer for use in formation of engineered animal skin, hide, and leather. Further described herein are methods of forming engineered animal skin, hide, and leather utilizing said layers of animal collagen-producing cells.
Claims29
A method of producing an engineered leather, the method comprising:
- culturing one or more types of collagen-producing cells in vitro;
- forming a plurality of sheets of extracellular matrix including collagen produced by the one or more types of collagen-producing cells;
- layering the plurality of sheets to form a body having a volume;
- allowing the layered sheets to fuse;
- and
- processing the body by tanning to modify the collagen.
A method of producing an engineered leather, the method comprising:
- culturing one or more types of collagen-producing cells in vitro;
- forming a plurality of sheets comprising the one or more types of collagen-producing cells and extracellular matrix including collagen produced by the one or more types of collagen-producing cells;
- stacking the sheets by layering the plurality of sheets atop each other to form a body having a volume;
- allowing the sheets to fuse;
- and
- processing the body by tanning to modify the collagen.
A method of making an engineered leather, the method comprising:
- culturing a first group of collagen-releasing cells to form a plurality of sheets of collagen;
- treating the plurality of sheets to prevent contractions thereby forming a plurality of non-contractile sheets;
- placing a second group of collagen-releasing cells on a first sheet from the plurality of non-contractile sheets;
- placing a second sheet from the plurality of non-contractile sheets on top of the first sheet to form a first stack;
- and
- culturing the first stack until the first sheet and second sheet are adherent.
A method of making an engineered leather, the method comprising:
- culturing collagen-releasing cells to confluency to form a plurality of sheets of collagen;
- decellularizing the plurality of sheets to prevent contractions;
- seeding collagen-releasing cells on a first sheet from the plurality of decellularized sheets;
- placing a second sheet from the plurality of decellularized sheets on top of the first sheet to form a stack;
- and
- culturing the stack until the first sheet and second sheet are adherent.
Dependencies are inferred from the claim text.