Defined glycoprotein products and related methods
AssigneeMomenta Pharmaceuticals, Inc.
InventorsBrian Edward Collins, Carlos J. Bosques, Lakshmanan Thiruneelakantapillai, Dorota A. Bulik, Ian C. Parsons, Zachary Shriver, Rajeev Chillakuru, Ganesh Kaundinya
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Abstract
The invention provides methods, databases and systems for making glycoprotein products having defined properties.
Claims7
-8. (canceled)
A method of producing a protein with a modulated amount of a glycan characteristic by
- modulating a production parameter, said method comprising:
- selecting a reference level of said glycan characteristic;
- selecting a value for a production parameter to provide a modulated level of said glycan characteristic;
- and
- applying the selected value of said production parameter in a process for making a protein with a modulated amount of said glycan characteristic, wherein said process for making the protein comprises culturing a CHO cell in a cell culture medium;
- wherein:
- said glycan characteristic is fucosylation and said modulated amount of said glycan characteristic is a decreased level of fucosylation;
- and
- said production parameter is the glucosamine content of the cell culture medium and said value for said production parameter is a glucosamine concentration of 0.001 to 40 mM.
The method of claim 12, wherein glucosamine is added to the cell culture medium.
The method of claim 12, wherein glucosamine is batch fed to the cell.
The method of claim 1, wherein an enzyme and/or substrate is added to the cell culture medium or batch fed to the cell such that glucosamine is produced.
The method of claim 15, wherein the substrate is selected from:
- N-acetylglucosamine, N-acetylglucosamine 6-phosphate, N-acetylmannosamine, and fructose.
The method of claim 1, wherein the protein is an antibody.
Dependencies are inferred from the claim text.