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US 2016/0097072 A1Published application
Non-caloric sweeteners and methods for synthesizing
AssigneeConagen Inc.
InventorsGuohong Mao, Yang Luo, Ying Lin, Xiaodan Yu
Life of this patent
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Abstract
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebaudioside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Claims22
A method for synthesizing rebaudioside E from rebaudioside KA, the method comprising:
- preparing a reaction mixture comprising rebaudioside KA, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and a UDP-glycosyltransferase selected from the group consisting of HV1, EUGT11 and a UDP-glycosyltransferase fusion enzyme;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside E, wherein a glucose is covalently coupled to the C2′ 13-O-glucose of rebaudioside KA to produce rebaudioside E.
-14. (canceled)
A method for synthesizing rebaudioside D2 from rubusoside, the method comprising:
- preparing a reaction mixture comprising rubusoside, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), a UDP-glycosyltransferase selected from the group consisting of EUGT11 and a UDP-glycosyltransferase fusion enzyme (EUS);
- and optionally, a sucrose synthase;
- incubating the reaction mixture for a sufficient time to produce a mixture of stevioside and rebaudioside KA, wherein a glucose is covalently coupled to the rubusoside to produce a mixture of stevioside and rebaudioside KA;
- further incubating the mixture of stevioside and rebaudioside KA with EUGT11 to produce rebaudioside E, wherein a glucose is covalently coupled to the stevioside and the rebaudioside KA to produce rebaudioside E;
- and
- further incubating the rebaudioside E with EUGT11 to produce rebaudioside D2, wherein a glucose is covalently coupled to the rebaudioside E to produce rebaudioside D2.
3 more elements
A method for synthesizing rebaudioside D2 from rebaudioside KA, the method comprising:
- preparing a reaction mixture comprising rebaudioside KA, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP)- and uridine diphosphate-glucose (UDP-glucose), a UDP-glycosyltransferase selected from the group consisting of an EUGT11 and a UDP-glycosyltransferase fusion enzyme (EUS);
- and optionally, a sucrose synthase;
- incubating the reaction mixture for a sufficient time to produce rebaudioside D2, wherein a glucose is covalently coupled to the rebaudioside KA to produce rebaudioside E;
- and
- further incubating the produced rebaudioside E with EUGT11 to produce rebaudioside D2.
2 more elements
Dependencies are inferred from the claim text.