Non-caloric sweeteners and methods for synthesizing
AssigneeConagen Inc.
InventorsGuohong Mao, Yang Luo, Ying Lin, Xiaodan Yu
Life of this patent
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Abstract
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Claims80
A synthetic rebaudioside consisting of a chemical structure:
A synthetic rebaudioside consisting of a chemical structure:
A method for synthesizing rebaudioside V from rebaudioside G, the method comprising:
- preparing a reaction mixture comprising rebaudioside G, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), a HV1 UDP-glycosyltransferase;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside V, wherein a glucose is covalently coupled to the rebaudioside G to produce rebaudioside V.
A method for synthesizing rebaudioside V from rebaudioside G, the method comprising:
- preparing a reaction mixture comprising rebaudioside G, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and a uridine dipospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of an EUGT11 and a UDP-glycosyltransferase fusion enzyme;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside V, wherein a glucose is covalently coupled to the rebaudioside G to produce rebaudioside V.
A method for synthesizing rebaudioside V from rebaudioside KA, the method comprising:
- preparing a reaction mixture comprising rebaudioside KA, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), a uridine dipospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of a UGT76G1 and a UDP-glycosyltransferase fusion enzyme;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside V, wherein a glucose is covalently coupled to the rebaudioside KA to produce rebaudioside V.
A method for synthesizing rebaudioside V from rubusoside, the method comprising:
- preparing a reaction mixture comprising rubusoside, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), uridine dipospho glycosyltransferases (UDP-glycosyltransferase) selected from the group consisting of a UGT76G1, HV1 and a UDP-glycosyltransferase fusion enzyme;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside V.
A method for synthesizing a rebaudioside V from rubusoside, the method comprising:
- preparing a reaction mixture comprising rubusoside, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), uridine dipospho glycosyltransferases (UDP-glycosyltransferase) selected from the group consisting of a UGT76G1, EUGT11 and a UDP-glycosyltransferase fusion enzyme;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside V.
A method for synthesizing rebaudioside W from rebaudioside V, the method comprising:
- preparing a reaction mixture comprising rebaudioside V, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), a uridine dipospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of a UGT76G1 and a UDP-glycosyltransferase fusion enzyme;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside W, wherein a glucose is covalently coupled to the rebaudioside V to produce rebaudioside W.
A method for synthesizing rebaudioside W from rebaudioside G, the method comprising:
- preparing a reaction mixture comprising rebaudioside G, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose);
- uridine dipospho glycosyltransferases (UDP-glycosyltransferase) selected from the group consisting of a UGT76G1, a UDP-glycosyltransferase fusion enzyme and a HV1;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside W, wherein a glucose is covalently coupled to the rebaudioside G to produce rebaudioside V by HV1, and a glucose is covalently coupled to the rebaudioside V to produce rebaudioside W by UGT76G1.
A method for synthesizing rebaudioside W from rebaudioside G, the method comprising:
- preparing a reaction mixture comprising rebaudioside G, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), uridine diphospho glycosyltransferases (UDP-glycosyltransferase) selected from the group consisting of an UGT76G1, an EUGT11, and a uridine diphospho glycosyltransferase fusion enzyme;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside W, wherein a glucose is covalently coupled to the rebaudioside G to produce rebaudioside V by EUGT11, and a glucose is covalently coupled to the rebaudioside V to produce rebaudioside W by UGT76G1.
A method for synthesizing rebaudioside W from rebaudioside KA, the method comprising:
- preparing a reaction mixture comprising rebaudioside KA, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), a uridine dipospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of a UGT76G1 and a UDP-glycosyltransferase fusion enzyme;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside W, wherein a glucose is covalently coupled to the rebaudioside KA to produce rebaudioside V, and a glucose is covalently coupled to the rebaudioside V to produce rebaudioside W.
A method for synthesizing a mixture rebaudioside W from rubusoside, the method comprising:
- preparing a reaction mixture comprising rubusoside, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), uridine diphospho glycosyltransferases (UDP-glycosyltransferase) selected from the group consisting of an UGT76G1, an HV1, and a UDP-glycosyltransferase-Sucrose synthase fusion enzyme;
- and
- incubating the reaction mixture for a sufficient time to produce rebaudioside W.
A method of producing of rebaudioside W from rubusoside, the method comprising:
- preparing a reaction mixture comprising rubusoside, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose);
- uridine dipospho glycosyltransferases (UDP-glycosyltransferase) selected from the group consisting of an UGT76G1, an EUGT11, and a UDP-glycosyltransferase synthase fusion enzyme, and
- incubating the reaction mixture for a sufficient time to produce rebaudioside W.
A sweetener consisting of a chemical structure:
A sweetener consisting of a chemical structure:
Dependencies are inferred from the claim text.