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US 2016/0097065 A1Published application
Methods of making surfactant and cleaning compositions through microbially produced branched fatty alcohols
AssigneeLS9, Inc.
InventorMathew RUDE
Life of this patent
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Abstract
The invention provides a surfactant and/or a cleaning composition comprising a microbially produced branched fatty alcohol or a derivative thereof. The invention also provides a household cleaning composition and a personal or pet care cleaning composition comprising a microbially produced branched fatty alcohol or a derivative thereof.
Claims11
1-31. (canceled)
32:
- A method of making a surfactant composition using branched chain fatty alcohols produced in a recombinant host cell, the method comprising,
- (a) providing a recombinant host cell genetically modified to comprise
- (i) a polynucleotide encoding a polypeptide comprising one or more subunits having branched chain alpha-keto acid dehydrogenase (BKD) activity (E.C. 1.2.4.4.) capable of catalyzing a conversion of an alpha-keto acid to a branched acyl-CoA,
- (ii) a polynucleotide encoding a polypeptide having beta-ketoacyl-ACP synthase (FabH) activity capable of catalyzing a condensation of a branched acyl-CoA and a malonyl-ACP to produce a branched acyl-ACP, and
- (iii) a polynucleotide encoding a polypeptide having fatty aldehyde biosynthesis activity capable of catalyzing a conversion of a branched fatty acyl-ACP into a branched fatty aldehyde;
- (b) culturing the recombinant host cell in the presence of a carbon source under conditions effective to express the polynucleotides and produce branched chain fatty alcohols that are secreted into the extracellular environment of the host cell;
- (c) collecting the branched chain fatty alcohols;
- and
- (d) blending the branched chain fatty alcohols to make a surfactant composition.
6 more elements
33:
- The method of claim 32, further comprising a polypeptide having fatty alcohol biosynthesis activity, wherein said polypeptide is an alcohol dehydrogenase (EC 1.1.1.1).
(canceled)
34:
- The method of claim 32, wherein said one or more subunits are selected from the group consisting of E1 alpha/beta (decarboxylase), E2 (dihydrolipoyl transacylase), and E3 (dihydrolipoyl dehydrogenase) subunits.
35:
- The method of claim 32, wherein said one or more subunits are selected from the group consisting of E1 alpha/beta (decarboxylase) and E2 (dihydrolipoyl transacylase) subunits.
36:
- The method of claim 32, wherein said polypeptide having fatty aldehyde biosynthesis activity is an acyl-ACP reductase (AAR).
37:
- The method of claim 36, wherein said AAR is an enzyme from Synechococcus elongates.
38:
- The method of claim 32, wherein said polypeptide having fatty aldehyde biosynthesis activity is carboxylic acid reductase (CAR).
39:
- The method of claim 32, wherein the recombinant host cell is an E. coli host cell.
40:
- The method of claim 32, wherein the branched chain fatty alcohols include one or more of saturated or unsaturated C12, C14 and C16 fatty alcohols.
Dependencies are inferred from the claim text.