Production Of Enantiopure alpha-Hydroxy Carboxylic Acids From Alkenes By Cascade Biocatalysis
AssigneeNational University of Singapore
InventorsZhi Li, Shuke Wu
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Abstract
The invention provides compositions comprising an alkene epoxidase and a selective epoxide hydrolase, such as a recombinant microorganism comprising a first heterologous nucleic acid encoding an alkene epoxidase and a second heterologous nucleic acid encoding a selective epoxide hydrolase. Exemplary alkene epoxidases include StyAB, while exemplary selective epoxide hydrolases include epoxide hydrolases from Sphingomonas, Solanum tuberosum, or Aspergillus. The invention also provides non-toxic methods of making enantiomerically pure vicinal diols or enantiomerically pure alpha-hydroxy carboxylic acids using these compositions and microorganisms.
Claims20
A composition comprising an alkene epoxidase and a selective epoxide hydrolase, wherein the composition is in the form of:
- a) a recombinant microorganism expressing the alkene epoxidase and selective epoxide hydrolase;
- b) a protein extract of the microorganism of a);
- c) purified alkene epoxidase and purified selective epoxide hydrolase;
- d) purified alkene epoxidase and purified selective epoxide hydrolase, wherein the purified enzymes are attached to solid supports;
- e) a composition of any one of a)-d), further comprising a diol oxidation system;
- or
- f) any combination of the foregoing.
A recombinant microorganism, comprising a first heterologous nucleic acid encoding an alkene epoxidase and a second heterologous nucleic acid encoding a selective epoxide hydrolase.
Dependencies are inferred from the claim text.