Production of Vectors for Non-Dividing Host Cells
AssigneeFinVector Vision Therapies Ltd.
InventorsHanna P. LESCH, Kari J. Airenne, Seppo Yla-Herttuala
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Abstract
A high-volume gene therapy vector manufacturing process, entailing using a recombinant baculovirus to transform a producer cell, which producer cell in turn produces a recombinant gene therapy vector which is able to transform host cells even when they are not dividing.
Claims17
A method comprising:
- a. Transducing a mammalian cell with a baculovirus to make a transduced mammalian producer cell;
- and then
- b. Culturing said transduced mammalian producer cell in culture media, and harvesting from said transduced mammalian producer cell and/or culture media a second virus having a therapeutic transgene.
A recombinant baculovirus having at least one nucleic acid sequence coding for a second virus derived from a virus which in its wild state has a single stranded genome, said second virus being replication-deficient and having a therapeutic transgene.
A viral vector able to transfect a human cell which is not actively dividing, said viral vector produced by a mammalian producer cell transduced with a recombinant baculovirus.
Dependencies are inferred from the claim text.