Enhanced expression and stability regions
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsGANG CHEN, ROBERT BABB, JAMES P. FANDL
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Abstract
Expression-enhancing nucleotide sequences for expression in eukaryotic systems are provided that allow for enhanced and stable expression of recombinant proteins in eukaryotic cells. Enhanced expression and stability regions (EESYRs) are provided for expression of a gene of interest in a eukaryotic cell. Chromosomal loci, sequences, and vectors are provided for enhanced and stable expression of genes in eukaryotic cells.
Claims34
A method of making a recombinant cell comprising introducing at least one exogenous sequence into an expression-enhancing expression and stability region (EESYR) sequence, or 5′ or 3′ of an EESYR sequence, wherein the EESYR sequence is selected from the group consisting of SEQ ID NO:1-6.
The method of claim 22, wherein the promoter comprises a eukaryotic promoter, and the eukaryotic promoter is operably linked to a prokaryotic operator.
A vector for use in making a recombinant cell, wherein the vector comprises a sequence homologous to an expression-enhancing expression and stability region (EESYR) sequence selected from the group consisting of SEQ ID NO:1-6, or a fragment thereof, and at least one exogenously added gene.
The vector of claim 24, wherein the EESYR sequence is selected from the group consisting of SEQ ID NO:1, 2, 3, 4, and a combination thereof.
The vector of claim 24, wherein the vector comprises a 5′ homology arm and a 3′ homology arm, wherein each homology arm is homologous to a sequence within any one of SEQ ID NO:1-6.
The vector of claim 24, wherein the vector comprises one, two, three, four, or five or more exogenously added genes.
Dependencies are inferred from the claim text.