Root-preferred promoter and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsANDREW CARL CROW, Scott Henry Diehn, Brooke Douglas Peterson-Burch
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Abstract
The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding Sorghum bicolor TIP2-3. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.
Claims20
An isolated nucleic acid molecule comprising a nucleotide sequence characterized by at least one of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 1 or a complement thereof;
- and
- c) a nucleotide sequence comprising at least 20 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1, wherein said sequence initiates transcription in a plant cell.
A method for expressing a nucleotide sequence in a plant or a plant cell, said method comprising introducing into the plant or the plant cell an expression cassette comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence characterized by at least one of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 1;
- and,
- b) a nucleotide sequence comprising at least 20 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1, wherein said nucleotide sequence initiates transcription in said plant.
A method for expressing a nucleotide sequence in a root-preferred manner in a plant, said method comprising introducing into a plant cell an expression cassette, and regenerating a plant from said plant cell, said plant having stably incorporated into its genome the expression cassette, said expression cassette comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 1;
- and
- b) a nucleotide sequence comprising at least 20 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1, wherein said sequence initiates transcription in a plant root cell.
Dependencies are inferred from the claim text.