Yeast promoters from pichia pastoris
AssigneeKeck Graduate Institute of Applied Life Sciences
InventorsIlya I. TOLSTORUKOV, James M. CREGG
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Abstract
In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated from Pichia pastoris wherein the nucleic acids have promoter activity are described. The invention also relates to expression methods, host cells, expression vectors, and DNA constructs, for using the Pichia pastoris promoters to produce proteins, and to the proteins produced using the expression methods.
Claims21
An isolated nucleic acid wherein the sequence of the isolated nucleic acid comprises a sequence at least 90% identical to a sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2, or at least 90% identical to a fragment thereof, wherein the isolated nucleic acid comprises the sequence of a constitutive Pichia pastoris promoter 1 operably linked to a heterologous coding sequence.
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Dependencies are inferred from the claim text.