Methods and Devices for In Situ Nucleic Acid Synthesis
AssigneeGen9, Inc.
InventorsJoseph Jacobson, Senthil Ramu, Daniel Schindler
Life of this patent
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Abstract
Disclosed are compositions, methods and devices for the in situ synthesis of nucleic acids. In an exemplary embodiment, a support-bound oligonucleotide is elongated by addition of one or more nucleotides by hybridization of a partially double-stranded oligonucleotide, ligation and removal of unwanted nucleotides.
Claims20
A composition for synthesizing a plurality of target nucleic acids on a surface of a solid support, the composition comprising a plurality of partially double-stranded oligonucleotides, each having a first strand and a second strand such that each partially double-stranded oligonucleotide comprises a double-stranded portion and a 5′ overhang on the second strand, wherein the double-stranded portion comprises at least one predetermined addition nucleotide at the 3′ end of the first strand, and wherein the double-stranded portion has a predefined sequence that is identical across the plurality of partially double-stranded oligonucleotides.
A composition for synthesizing a plurality of target nucleic acids on a surface of a solid support, the composition comprising:
- a support having a plurality of features, each feature comprising a plurality of single-stranded support-bound oligonucleotides;
- and
- a first partially double-stranded oligonucleotide comprising a first strand and a second strand forming a double-stranded portion and a 5′ overhang on the second strand, wherein the first strand comprises a first predetermined ligatable addition nucleotide at the 3′ end;
- wherein the first partially double-stranded oligonucleotide can be ligated to a first support-bound oligonucleotide at the first predetermined ligatable addition nucleotide, thereby generating a first ligation product comprising the first predetermined addition nucleotide;
- and wherein the first ligation product is cleavable thereby generating a first elongated support-bound oligonucleotide comprising the first predetermined addition nucleotide.
Dependencies are inferred from the claim text.