Process for manufacturing a composite sorbent material for chromatographical separation of biopolymers

A process for the purification and/or isolation of nucleic acids wherein the process comprises

• lysing cells yielding a sample containing nucleic acids and proteins,
• contacting the sample with a sorbent material binding the proteins, and
• collecting the eluate containing the nucleic acids
• where the sorbent material comprises a porous inorganic material comprising silica which is at least partially covered by a polymer coating having the general formula
• wherein the mass ratio of copolymer components [A], [B], and [M] complies with the formula 1:0.03-0.3:0-0.2 and
• [A] is derived from a monomer with general formula
• R═H;
• 2, 3, 4 —OH;
• —COCH;
• —NO2;
• —Cl;
• —Br;
• —F;
• —OAlk;
• —OAc;
• —NH2;
• —NHAc;
• —N(Alk)2;
• -Alk (C1-C16)
• [B] is derived from a monomer of general formula
• R═H;
• 2, 3, —OH;
• —COOH;
• —NO2;
• —Cl;
• —Br, —F;
• —OAlk;
• —OAc;
• —NH2;
• —NHAc;
• —N(Alk)2;
• -Alk (C1-C15);
• and
• [M] is derived from a monomer of general formula
• R═H;
• Ar;
• Alk (C1-C12)
• R1═H;
• Ar;
• Alk (C1-C12);
• —(—CH2—)n—OH n=2-18
• or a monomer of general formula
• n=1-18
• R═H;
• Ar;
• Alk, (C1-C12)
• R1═H;
• Ar;
• Alk.(C1-C12);
• CH2mOH m=2 -18