Nitrile hydratase

A modified Rhodococcus bacterial or Nocardia bacterial nitrile hydratase, comprising in the α subunit,

• a) an amino-acid sequence as shown in SEQ ID NO:
• 119:
• AX1X2X3X4GX5X6GX7X8,
• (SEQ ID NO:
• 119)
• wherein
• A is alanine,
• G is glycine,
• X1˜X7 each independently indicate any amino-acid residue, and
• X8 is an amino acid selected from the group consisting of alanine, leucine, methionine, asparagine, cysteine, aspartic acid, glutamic acid, phenylalanine, glycine, histidine, lysine, proline, arginine, serine, threonine and tryptophan;
• b) an amino-acid sequence as shown in SEQ ID NO:
• 132:
• AX1X2X3X4GX5X6GX7Q,
• (SEQ ID NO:
• 132)
• wherein
• A is alanine,
• G is glycine,
• Q is glutamine,
• X1˜X6 each independently indicate any amino-acid residue, and
• X7 is substituted with an amino acid different from that in a wild type;
• or
• (c) an amino-acid sequence as shown in SEQ ID NO:
• 136:
• AX1X2X3X4GX5X6GX7QX8X9,
• (SEQ ID NO:
• 136)
• wherein
• A is alanine,
• G is glycine,
• Q is glutamine,
• X1˜X8 each independently indicate any amino-acid residue, and
• X9 is substituted with an amino acid different from that in a wild type.