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US 2016/0097045 A1Published application
Process and system for obtaining botulinum neurotoxin
AssigneeAllergan, Inc.
InventorsJennifer L. Ton, Hemant A. Patel, Ronald C. Bates, Wajdie M. Ahmad
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Abstract
Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.
Claims9
A substantially animal product free (APF) process utilizing chromatography for obtaining a botulinum neurotoxin, the process comprising the following steps:
- (a) providing a substantially APF fermentation medium;
- (b) fermenting clostridium botulinum bacteria in the fermentation medium, wherein the clostridium botulinum bacteria produce a botulinum neurotoxin, and;
- (c) sequentially contacting a plurality of chromatography resins with an aqueous medium containing the botulinum neurotoxin, wherein the plurality of chromatography resins comprise an ion exchange resin, a hydrophobic interaction resin, a gel filtration resin, or combinations thereof;
- thereby obtaining the botulinum neurotoxin from the substantially APF chromatography process.
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Dependencies are inferred from the claim text.