Method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby
AssigneeThe Trustees of the University of Pennsylvania
InventorsGuangping Gao, James M. Wilson, Mauricio R. Alvira
Life of this patent
Estimating the term…
Abstract
Adeno-associated virus rh.8 sequences, vectors containing same, and methods of use are provided.
Claims35
A recombinant adeno-associated virus (AAV) comprising an AAVrh.8 capsid comprising vp1, vp2 and vp3 proteins, wherein at least one vp1 has the sequence of amino acids 1 to 736 of SEQ ID NO:97 or a sequence at least 95% identical to SEQ ID NO:
- 97, said AAV having packaged in the capsid a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR), and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression of the product in a host cell.
A recombinant adeno-associated virus (AAV) comprising an AAVrh.8 capsid comprising vp1, vp2 and vp3 proteins, wherein at least one vp2 has the amino acid sequence of 138 to 736 of SEQ ID NO:
- 97 or a sequence at least 95% identical to amino acid 138 to 736 of SEQ ID NO:
- 97, said AAV having packaged in the capsid a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression thereof in a host cell.
A recombinant adeno-associated virus (AAV) comprising an AAVrh.8 capsid comprising vp1, vp2 and vp3 proteins, wherein at least one vp3 has the amino acid sequence of 203 to 736 of SEQ ID NO:
- 97 or a sequence at least 95% identical to amino acid 203 to 736 of SEQ ID NO:
- 97, said AAV having packaged in the capsid a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression of the product in a host cell.
A method of generating a recombinant adeno-associated virus (AAV) comprising an AAV rh.8 capsid comprising culturing a host cell containing:
- (a) a molecule encoding the AAVrh.8 vp1 capsid protein having a sequence of amino acids 1 to 736 of SEQ ID NO:
- 97, or a sequence which is at least 95% identical to the full length of SEQ ID NO:
- 97;
- (b) a functional rep gene;
- (c) a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression of the product in a host cell;
- and (d) sufficient helper functions to permit packaging of the minigene into the AAV capsid protein under conditions which permit packaging of the minigene into the AAV capsid.
A molecule comprising a nucleic acid sequence encoding a vp2 capsid protein having the sequence of amino acids (aa) 138 to 736 of SEQ ID NO:
- 97 or a vp3 capsid protein having the sequence of amino acids 203 to 737 of SEQ ID NO:
- 97 operably linked to heterologous expression control sequences which direct expression thereof in a packaging host cell.
A molecule comprising a nucleic acid sequence encoding a novel adeno-associated virus (AAV) rh.8 capsid protein having an amino acid sequence 1 to 737 of SEQ ID NO:
- 97 operably linked to heterologous expression control sequences which direct expression thereof in a packaging host cell.
A recombinant adeno-associated virus (AAV) comprising an AAV capsid having an amino acid sequence selected from the group consisting of:
- C1, SEQ ID NO:60;
- C2, SEQ ID NO:61;
- C5, SEQ ID NO:62;
- A3-3, SEQ ID NO:66;
- A3-7, SEQ ID NO:67;
- A3-4, SEQ ID NO:68;
- A3-5, SEQ ID NO:
- 69;
- 3.3b, SEQ ID NO:
- 62;
- 223.4, SEQ ID NO:
- 73;
- 223-5, SEQ ID NO:74;
- 223-10, SEQ ID NO:75;
- 223-2, SEQ ID NO:76;
- 223-7, SEQ ID NO:
- 77;
- 223-6, SEQ ID NO:
- 78;
- 44-1, SEQ ID NO:
- 79;
- 44-5, SEQ ID NO:80;
- 44-2, SEQ ID NO:81;
- 42-15, SEQ ID NO:
- 84;
- 42-8, SEQ ID NO:
- 85;
- 42-13, SEQ ID NO:86;
- 42-3A, SEQ ID NO:87;
- 42-4, SEQ ID NO:88;
- 42-5A, SEQ ID NO:89;
- 42-1B, SEQ ID NO:90;
- 42-5B, SEQ ID NO:91;
- 43-1, SEQ ID NO:
- 92;
- 43-12, SEQ ID NO:
- 93;
- 43-5, SEQ ID NO:94;
- 43-21, SEQ ID NO:96;
- 43-20, SEQ ID NO:99;
- 24.1, SEQ ID NO:
- 101;
- 42.2, SEQ ID NO:102;
- 7.2, SEQ ID NO:
- 103;
- 27.3, SEQ ID NO:
- 104;
- 16.3, SEQ ID NO:
- 105;
- 42.10, SEQ ID NO:
- 106;
- 42-3B, SEQ ID NO:
- 107;
- 42-11, SEQ ID NO:
- 108;
- F1, SEQ ID NO:
- 109;
- F5, SEQ ID NO:
- 110;
- F3, SEQ ID NO:111;
- 42-6B, SEQ ID NO:
- 112;
- and 42-12, SEQ ID NO:
- 113,
- said AAV having packaged in the capsid a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR), and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression of the product in a host cell.
Dependencies are inferred from the claim text.