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AssigneesKyushu University, National University Corporation, Tella, Inc.
InventorsYoshikazu Yonemitsu, Satoru Saito, Yui Harada, Yuichiro Yazaki, Takefumi Ishidao
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The object of the present invention is to develop a technique in which NK cells having a high cytotoxic activity can be prepared with high purity from hematopoietic precursor cells without using a serum or feeder cells of an animal. A method for expanding NK cells includes the steps of expanding hematopoietic precursor cells under a single culturing condition using a medium supplemented with IL-15, SCF, IL-7 and Flt3L, and differentially inducing the cells obtained in the expanding step into NK cells under a culturing condition using a medium supplemented with IL-2. A pharmaceutical composition contains the NK cells prepared by the method for expanding NK cells of the present invention. The pharmaceutical composition of the present invention is used for treating an infectious disease and/or a cancer.
1-6. (canceled)
A method for preparing NK cells, comprising the steps of
The method for preparing NK cells according to 8, wherein the medium used in each of the steps is supplemented with a human AB-type serum and/or a human serum albumin.
Dependencies are inferred from the claim text.