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US 2016/0097021 A1Published application
Cleaning agent containing proteases
AssigneeHenkel AG & Co. KGaA
InventorsThomas Eiting, Nina Mussmann, Thorsten Bastigkeit, Noelle Wrubbel
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Abstract
The present invention relates to a cleaning agent having improved cleaning performance wherein the agent comprises a first protease and a second protease and to a cleaning method, particularly an automatic cleaning method in which this agent is used.
Claims10
A cleaning agent, characterized by comprising at least one first protease and one second protease, wherein
- a) the first protease comprises an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 1 over the entire length thereof and includes at least one amino acid substitution at one of the positions 9, 15, 66, 212 and 239 using the numbering according to SEQ ID NO. 1, and
- b) the second protease is selected from the group consisting of:
- i) protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 2 over the entire length thereof and includes the amino acid glutamic acid (E) at position 99 using the numbering according to SEQ ID NO. 2;
- ii) protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 2 over the entire length thereof and includes the amino acid substitution R99E using the numbering according to SEQ ID NO. 2 in combination with at least two further amino acid substitutions, which are selected from the group consisting of S3T, V4I, and V199I;
- and
- iii) protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 1 over the entire length thereof and includes the amino acid substitution S99A using the numbering according to BPN′ in combination with an insertion between positions 99 and 100, wherein the inserted amino acid is aspartic acid (D).
3 more elements
Dependencies are inferred from the claim text.