Antibodies to masp-2
AssigneesHelion Holding ApS, Helion Biotech ApS
InventorsFlemming Larsen, Ulla Wahlers
Life of this patent
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Abstract
The invention relates to antibodies to MASP-2 and functional equivalents thereof. In particular, the invention relates to MASP-2 antibodies capable of inhibiting the function of MASP-2. The invention furthermore discloses MASP-2 epitopes, wherein antibodies recognising said epitopes are in particularly useful for inhibiting MASP-2 activity. The invention also relates to methods of producing said antibodies, methods of inhibiting MASP-2 activity as well as to pharmaceutical compositions comprising the MASP-2 antibodies.
Claims30
An antibody or antigen-binding fragment thereof specifically recognising and binding a polypeptide fragment of human MASP-2 consisting of the CCP1, CCP2 and serine protease domains (aa 293 to 686 of SEQ ID NO:1), wherein said antibody or antigen-binding fragment thereof is capable of displacing one or more reference antibodies in a competitive ELISA assay, wherein the one or more reference antibodies selected from the group consisting of:
- (i) the monoclonal antibody produced by the hybridoma cell line deposited under the deposition number 03050904;
- (ii) the monoclonal antibody produced by the hybridoma cell line deposited under the deposition number DSM ACC2657
- (iii) the monoclonal antibody produced by the hybridoma cell e deposited under the deposition number DSM ACC2660;
- (iv) the monoclonal antibody produced by the hybridoma cell line deposited under the deposition number DSM ACC2658;
- and
- (v) the monoclonal antibody produced by the hybridoma cell line deposited under the deposition number DSM ACC2659,
- wherein the antibody, or antigen-binding fragment thereof, is capable of inhibiting C4 deposition.
An antibody comprising a variable region of the heavy chain comprising a sequence which is at least 90% identical to SEQ ID NO:4, or an antigen binding fragment thereof, wherein said antibody or antigen-binding fragment thereof is capable of inhibiting C4 deposition.
An antibody comprising a variable region of the light chain comprising a sequence which is at least 90% identical to SEQ ID NO:5 or an antigen binding fragment thereof, wherein said antibody or antigen binding fragment thereof is capable of inhibiting C4 deposition.
A monoclonal antibody, or antigen-binding fragment thereof specifically recognizing and binding an epitope within the C-terminal part of MASP-2, wherein the antibody, or antigen-binding fragment thereof comprises the following CDRs:
- 1) CDR1 of the heavy chain of SEQ ID 6;
- 2) CDR2 of the heavy chain of SEQ ID 7;
- 3) CDR3 of the heavy chain of SEQ ID 8;
- 4) CDR1 of the light chain of SEQ ID 9;
- 5) CDR2 of the light chain of SEQ ID 10;
- and
- 6) CDR3 of the light chain of SEQ ID 11;
- wherein said antibody or antigen-binding fragment thereof is capable of inhibiting C4 deposition.
Dependencies are inferred from the claim text.