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US 2016/0096881 A1Published application
Means and methods for producing high affinity antibodies
AssigneeAimm Therapeutics B.V.
InventorsTim BEAUMONT, Mark Jeroen KWAKKENBOS, Hergen SPITS, Adrianus Quirinus BAKKER, Koen WAGNER
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Abstract
The invention provides means and methods for producing high-affinity antibodies against an antigen of interest, usually stable B-cell cultures.
Claims4
An isolated or recombinant antibody-producing B-cell produced by the method comprising:
- a) selecting a B-cell able to produce and antibody specific for an antigen of interest or selecting a B-cell able to differentiate into a B-cell that is able to produce antibody specific for said antigen of interest;
- b) inducing, enhancing and/or maintaining expression of BCL6 in said B-cell;
- c) inducing, enhancing and/or maintaining expression of an anti-apoptotic nucleic acid in said B-cell;
- d) allowing expansion of said B-cells into a first population of B-cells;
- e) performing antigen staining and BCR staining of B-cells from said first population;
- f) selecting a B-cell that has a high antigen affinity;
- g) allowing expansion of said B-cell into a second population of B-cells;
- h) measuring the antigen association rate and dissociation rate of antibodies produced by at least one B-cell from said second population;
- and
- i) selecting an antibody with an affinity for said antigen of interest that is higher than the average affinity of said first population of B-cells for said antigen of interest
7 more elements
A population of antibody-producing B-cells obtained by the method comprising:
- a) selecting a B-cell able to produce and antibody specific for an antigen of interest or selecting a B-cell able to differentiate into a B-cell that is able to produce antibody specific for said antigen of interest;
- b) inducing, enhancing and/or maintaining expression of BCL6 in said B-cell;
- c) inducing, enhancing and/or maintaining expression of an anti-apoptotic nucleic acid in said B-cell;
- d) allowing expansion of said B-cells into a first population of B-cells;
- e) performing antigen staining and BCR staining of B-cells from said first population;
- f) selecting a B-cell that has a high antigen affinity;
- g) allowing expansion of said B-cell into a second population of B-cells;
- h) measuring the antigen association rate and dissociation rate of antibodies produced by at least one B-cell from said second population;
- and
- i) selecting an antibody with an affinity for said antigen of interest that is higher than the average affinity of said first population of B-cells for said antigen of interest
7 more elements
Dependencies are inferred from the claim text.