Systems and methods for biological analysis

A method of processing a plurality of biological samples, comprising:

• providing a case comprising:
• a base;
• a cover disposed over at least a portion of the base, the base and the cover together forming an inner chamber;
• a substrate located within the inner chamber, the substrate comprising a first surface, an opposing second surface opposite the first surface, and a plurality of through-holes disposed between the surfaces, the plurality of through-holes containing one or more biological samples;
• a sealing fluid disposed within the inner chamber and covering the surfaces of the substrate;
• placing the base in thermal contact with a temperature controller;
• performing an assay or reaction on the one or more biological samples while maintaining or controlling a temperature of at least one of the support, the case, the base, the substrate, the cover, the sealing fluid, or the one or more biological samples;
• maintaining the surfaces of substrate at a positive angle relative to a horizontal plane while performing the assay or reaction.

A system for processing a plurality of biological samples, comprising:

• a support configured to hold a case comprising:
• an inner chamber;
• and
• a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples;
• and
• a temperature controller configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples;
• wherein the support is configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.