De novo synthesized gene libraries
AssigneeTwist Bioscience Corporation
InventorsWilliam BANYAI, Bill James PECK, Andres FERNANDEZ, Siyuan CHEN, Pierre INDERMUHLE
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Abstract
De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
Claims31
-20. (canceled)
A device for nucleic acid assembly, comprising:
- a silicon dioxide structure comprising a plurality of wells, wherein each well of the plurality of wells has height from 0.1 mm to 0.6 mm, and wherein each well of the plurality of wells has a cross-section from 0.8 to 2.0 mm;
- an inner region of each well of the plurality of wells, wherein the inner region comprises a high surface energy coating;
- an outer region of each well of the plurality of wells, wherein the outer region comprises a low surface energy coating, and wherein a difference in water contact angle between the inner region and the outer region is at least 10 degrees as measured on a planar surface.
A device for nucleic acid assembly, comprising:
- a structure comprising a plurality of wells, wherein each well of the plurality of wells has a height from 0.1 mm to 0.6 mm, and wherein each well of the plurality of wells has a cross-section from 0.8 to 2.0 mm;
- an inner region of each well of the plurality of wells, wherein the inner region comprises a hydrophilic coating;
- an outer region of each well of the plurality of wells, wherein the outer region comprises a hydrophobic coating, and wherein the hydrophobic coating has a surface energy lower than a surface energy of the hydrophilic coating;
- and
- an aqueous liquid comprising nucleic acid assembly reagents, wherein the aqueous liquid has a surface tension from 12 to 80 millinewtons per meter.
Dependencies are inferred from the claim text.