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AssigneeThe Johns Hopkins University
InventorsWARREN GRAYSON, COLIN COOK, BEN P.J. HUNG, PINAR HURI, DAPHNE L. HUTTON, JOSHUA TEMPLE
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The presently disclosed subject matter focuses on recapitulating the heterotypic interactions needed to maximize the co-development of vasculature and bone. More particularly, the presently disclosed subject matter explores the potential of cellular aggregation and temporal presentation of factors to induce the cell-cell signaling events required to stimulate ASCs to self-organize into vascularized bone. Further, exogenous PDGF-BB synergizes complex tissue formation in ASC cultures by enhancing vascular stability and osteogenic differentiation. The presently disclosed approach provides a robust protocol to engineer vascularized bone with ASCs in vitro.
A biodegradable scaffold for regenerating bone tissue, the scaffold configured to form a porous, three-dimensional (3D) network of interconnected void spaces, wherein the porous, three-dimensional network further comprises a hydrogel, and wherein the hydrogel comprises one or more cells encapsulated therein and one or more growth factors capable of promoting regeneration of bone tissue.
A method for preparing a composite for promoting regeneration of bone tissue, the method comprising:
A method for treating a bone defect, the method comprising contacting the bone defect with a biodegradable scaffold for regenerating bone tissue, the scaffold configured to form a porous, three-dimensional (3D) network of interconnected void spaces, wherein the porous, three-dimensional network of further comprise a hydrogel, and wherein the hydrogel comprises one or more cells encapsulated therein and one or more growth factors capable of promoting regeneration of bone tissue.
Dependencies are inferred from the claim text.