Enhanced loading of intact, bacterially derived vesicles with small molecule compounds
AssigneeEnGeneIC Molecular Delivery Pty Ltd
InventorsHimanshu Brahmbhatt, Jennifer MacDiarmid
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Abstract
Enhanced loading of small molecule compounds into intact, bacterially derived vesicles provides operational and therapeutic advantages.
Claims25
A composition comprising an intact and nonliving bacterial vesicle that encloses a fluorescent small molecule drug, which is not doxorubicin, irinotecan, bisantrene, topotecan, epirubicin, daunorubicin, mitoxantrone, Oregon Green® 488-conjugated paclitaxel, or BODIPY® FL-conjugated vinblastine.
A composition comprising an intact and nonliving bacterial vesicle that encloses a compound of formula D-L-F or a salt thereof, wherein D is the residue of a small molecule drug, L is a linker, and F is a fluorescent moiety.
A composition comprising an intact, bacterially derived bacterial vesicle enclosing a compound that comprises an active agent bound through a linker to an energy transfer moiety, wherein the active agent is other than Oregon Green® 488-conjugated paclitaxel and BODIPY® FL-conjugated vinblastine.
A method of loading a plurality of minicells with a desired compound without resort to centrifugation, comprising:
- (a) incubating the plurality in a volume of an incubation solution of the compound in a buffered liquid, wherein the volume is on the order of about 100 mls or more;
- and then
- (b) subjecting the plurality to multiple washing steps, each comprising cross-flow filtration of the minicells with a volume of buffered liquid that is on the order of liters, wherein none of the washing steps employs centrifugation of the minicells.
Dependencies are inferred from the claim text.