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US 2016/0095314 A1Published application

Hypersensitive response elicitor peptides and use thereof

AssigneePlant Health Care, Inc.

InventorsZhongmin Wei, Gregory A. Zornetzer, Stephen Bornick

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Abstract Drawings Claims Description

Abstract

Disclosed are hypersensitive-response eliciting peptides that exhibit improved solubility, stability, resistance to chemical degradation, or a combination of these properties. Use of these peptides or fusion polypeptides, or DNA constructs encoding the same, for modulating plant biochemical signaling, imparting disease resistance to plants, enhancing plant growth, imparting tolerance to biotic stress, imparting tolerance and resistance to abiotic stress, imparting desiccation resistance to cuttings removed from ornamental plants, imparting post-harvest disease or post-harvest desiccation resistance to a fruit or vegetable, or enhancing the longevity of fruit or vegetable ripeness are also disclosed.

Claims207

Dependent claims

Claim 1An isolated peptide comprising the amino acid sequence of (L/I/V/F)-X—X-(L/I/V/F)-(L/I)-X—X-(L/I/V/F)-(L/I/V/A)-X—X-(L/I)-(L/I/V/F) (SEQ ID NO · 53 words123 dependent Claim 18An isolated peptide comprising the amino acid sequence of XXGISEKXXXXXXXXXXXXXXXX (SEQ ID NO · 287 words121 dependent Claim 33An isolated peptide of less than 100 amino acids in length · 24 words109 dependent Claim 36An isolated peptide comprising the amino acid sequence of (i) KPXDSXSXIAKLISXLIXSLLX (SEQ ID NO · 190 words113 dependent Claim 43An isolated peptide · 33 words1 dependent Claim 44An isolated peptide · 13 words107 dependent Claim 45An isolated peptide comprising the amino acid sequence of (i) PSPXTXXLXXIVGXILXAXN (SEQ ID NO · 253 words118 dependent Claim 57An isolated peptide comprising the amino acid sequence of (i) LXXL(L/M)XILXXLV (SEQ ID NO · 244 words116 dependent Claim 67An isolated peptide comprising the amino acid sequence of (i) XXXXXXXXXXX(L/M)XXLLXXLLXXLLXXX (SEQ ID NO · 704 words115 dependent Claim 76An isolated peptide · 153 words113 dependent Claim 83An isolated peptide comprising the amino acid sequence of (i) LXXLLXXLVXLLK (SEQ ID NO · 265 words115 dependent Claim 92An isolated peptide comprising the amino acid sequence of (i) (L/M)XXLLX(L/M)FXXI(L/M)XX (SEQ ID NO · 139 words115 dependent

An isolated peptide comprising the amino acid sequence of

(L/I/V/F)-X—X-(L/I/V/F)-(L/I)-X—X-(L/I/V/F)-(L/I/V/A)-X—X-(L/I)-(L/I/V/F) (SEQ ID NO:
93)
wherein
the peptide is free of cysteine and methionine;
each X at positions 2, 6, and 10 is optional and, when present, is any amino acid;
and
each X at positions 3, 7, and 11 is any amino acid.

4 more elements

An isolated peptide comprising the amino acid sequence of

XXGISEKXXXXXXXXXXXXXXXX (SEQ ID NO:
1, P1/P4 consensus), wherein
X at position 1 is optional and can be S, N, D, isoD, G, A, or S;
X at position 2 is optional and can be Q, E, g-glutamate, G, A, or S;
X at position 8 is Q, E, g-glutamate, G, A, or S;
X at position 9 is L, I, F, or V;
X at position 10 is optional and can be D or isoD;
X at position 11 is Q, E, g-glutamate, G, A, or S;
X at position 12 is M, L, I, or F;
X at position 13 is M, L, or I;
X at position 14 is optional and can be any hydrophilic amino acid;
X at position 15 is Q, E, g-glutamate, G, A, S, K, or I;
X at position 16 is M, L, I, V, or F;
X at position 17 is M, L, I, A, or V;
X at position 18 is Q, E, g-glutamate, G, A, S, M, T, or K;
X at position 19 is A, D, isoD, S, V, T, K, R, E, H, or G;
X at position 20 is M, L, or I;
X at position 21 is M, L, I, V, S, or F;
X at position 22 is Q, E, g-glutamate, G, A, S;
X at position 23 is P, Q, E, g-glutamate, G, A, or S;
and
wherein the isolated peptide comprises one or more mutations, relative to a corresponding wildtype amino acid sequence, which one or more mutations improve the stability or resistance to chemical degradation of the isolated peptide relative to a polypeptide comprising the corresponding wildtype amino acid sequence.

19 more elements

An isolated peptide of less than 100 amino acids in length, which isolated peptide comprises the amino acid sequence of SEQ ID NO:

5.

An isolated peptide comprising the amino acid sequence of

(i) KPXDSXSXIAKLISXLIXSLLX (SEQ ID NO:
47, P15b/P20 consensus), wherein
X at position 3 is N, D, or isoD;
X at position 6 is Q, E, g-glutamate, G, A, or S;
X at position 8 is N, D, or isoD;
X at position 15 is optional and can be any amino acid;
X at position 18 is M, E, g-glutamate, G, A, S, T, or K;
and
X at position 22 is optional and can be Q, E, g-glutamate, G, A, or S;
or
(ii) IAKLISXLIXSLLX (SEQ ID NO:
12, P15/20 min consensus), wherein
X at position 7 is optional and can be any amino acid;
X at position 10 is M, E, g-glutamate, G, A, S, T, or K;
and
X at position 14 is optional and can be Q, E, g-glutamate, G, A, or S.
wherein the isolated peptide comprises one or more mutations, relative to a corresponding wildtype amino acid sequence, which one or more mutations improve the stability or resistance to chemical degradation of the isolated peptide relative to a polypeptide comprising the corresponding wildtype amino acid sequence.

14 more elements

An isolated peptide comprising from 22 to 36 amino acids, wherein the peptide consists essentially of the amino acids of SEQ ID NO:

49, SEQ ID NO:
63, or SEQ ID NO:
64.

An isolated peptide comprising the amino acid sequence of SEQ ID NO:

65.

An isolated peptide comprising the amino acid sequence of

(i) PSPXTXXLXXIVGXILXAXN (SEQ ID NO:
66, peptide 6/6a consensus), wherein
X at position 4 is F or Y;
X at position 6 is Q, E, g-glutamate, G, A, or S;
X at position 7 is optional and can be M, E, g-glutamate, G, A, S, T, or K;
X at position 9 is M, E, g-glutamate, G, A, S, T, or K;
X at position 10 is H or N;
X at position 14 is E, g-glutamate, D, or isoD;
X at position 17 is Q, E, g-glutamate, G, A, or S;
and
X at position 19 is Q, E, g-glutamate, G, A, or S;
or
(ii) XTXXLXXIVGXIL (SEQ ID NO:
135, P6/6a min consensus), wherein
X at position 1 is F or Y;
X at position 3 is Q, E, g-glutamate, G, A, or S;
X at position 4 is optional and, according to one embodiment, can be M, E, g-glutamate, G, A, S, T, or K;
or according to another embodiment can be L;
X at position 6 is M, E, g-glutamate, G, A, S, T, or K;
X at position 7 is H or N;
and
X at position 11 is E, g-glutamate, D, or isoD;
wherein the isolated peptide comprises one or more mutations relative to a corresponding wildtype amino acid sequence, which one or more mutations improve the stability or resistance to chemical degradation of the isolated peptide relative to a polypeptide comprising the corresponding wildtype amino acid sequence.

20 more elements

An isolated peptide comprising the amino acid sequence of

(i) LXXL(L/M)XILXXLV (SEQ ID NO:
16, P25 consensus) wherein
X at position 2 can be Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
X at position 3 can be K, Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
X at position 6 can be K, Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
X at position 9 can be E, g-glutamate, D, isoD, Q, N, T, S, A, or G;
and
X at position 10 can be A, G, S, T, E, g-glutamate, D, isoD, Q, or N;
or
(ii) LXXVLXXL(L/M)XILXXLV (SEQ ID NO:
17, P25 consensus) wherein
X at position 2 can be T, S, A, G, D, isoD, E, g-glutamate, Q, or N;
X at position 3 can be G, T, S, A, D, isoD, E, g-glutamate, Q, or N;
X at position 6 can be Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
X at position 7 can be K, Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
X at position 10 can be K, Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
X at position 13 can be E, g-glutamate, D, isoD, Q, N, T, S, A, or G;
X at position 14 can be A, G, S, T, E, g-glutamate, D, isoD, Q, or N;
and
V at position 16 is optional.

17 more elements

An isolated peptide comprising the amino acid sequence of

(i) XXXXXXXXXXX(L/M)XXLLXXLLXXLLXXX (SEQ ID NO:
18, P17/18), wherein
X at position 1 can be any amino acid, but preferably Q, S, E, g-glutamate, A, T, G, D, isoD, N, K, or R;
X at position 2 can be any amino acid, but preferably Q, S, E, g-glutamate, A, T, G, D, isoD, N, K, or R;
X at position 3 can be any amino acid, but preferably P, Q, S, E, g-glutamate, A, T, G, D, isoD, N, K, or R;
X at position 4 can be any amino acid, but preferably I, Q, S, E, g-glutamate, A, T, G, D, N, isoD, K, or R;
X at position 5 can be any amino acid, but preferably D, isoD, S, E, g-glutamate, A, T, G, N, Q, K, or R;
X at position 6 can be any amino acid, but preferably R, Q, S, E, g-glutamate, A, T, G, D, isoD, N, or K;
X of position 7 can be any amino acid, but preferably Q, S, E, g-glutamate, A, T, G, D, isoD, N, K, or R;
X at position 8 can be any amino acid, but preferably T, Q, S, E, g-glutamate, A, G, D, isoD, N, K, or R;
X at position 9 can be any amino acid, but preferably I, Q, S, E, g-glutamate, A, T, G, D, isoD, N, K, or R;
X at position 10 can be any amino acid, but preferably E, g-glutamate, Q, S, A, T, G, D, isoD, N, K, or R;
X at position 11 can be any amino acid, but preferably Q, S, E, g-glutamate, A, T, G, D, isoD, N, K, or R;
X at position 13 can be any amino acid, but preferably A, S, T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
X at position 14 can be any amino acid, but preferably Q, A, S, T, G, D, isoD, E, g-glutamate, N, K, or R;
X at position 17 can be any amino acid, but preferably A, S, T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
X at position 18 can be any amino acid, but preferably Q, A, S, T, G, D, isoD, E, g-glutamate, N, K, or R;
X at position 21 can be any amino acid, but preferably K, A, S, T, G, D, isoD, E, g-glutamate, Q, N, or R;
X at position 22 can be any amino acid, but preferably S, A,T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
X at position 25 can be any amino acid, but preferably S, A, T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
X at position 26 can be any amino acid, but preferably P, S, A, T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
and
X at position 27 can be any amino acid, but preferably Q, S, A, T, G, D, isoD, E, g-glutamate, N, K, or R;
or
(ii) (L/M)XXLLXXLLXXLL (SEQ ID NO:
25, P17/18 min consensus), wherein
X at position 2 can be any amino acid, but preferably A, S, T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
X at position 3 can be any amino acid, but preferably Q, A, S, T, G, D, isoD, E, g-glutamate, N, K, or R;
X at position 6 can be any amino acid, but preferably A, S, T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
X at position 7 can be any amino acid, but preferably Q, A, S, T, G, D, isoD, E, g-glutamate, N, K, or R;
X at position 10 can be any amino acid, but preferably K, A, S, T, G, D, isoD, E, g-glutamate, Q, N, or R;
and
X at position 11 can be any amino acid, but preferably S, A,T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
wherein the isolated peptide comprises one or more mutations relative to a corresponding wildtype amino acid sequence, and the one or more mutations improve the aqueous solubility, stability, or resistance to chemical degradation of the isolated peptide relative to a polypeptide comprising the corresponding wildtype amino acid sequence.

31 more elements

An isolated peptide comprising the amino acid sequence of XLXX(L/M)LXLIXX(L/I/V/F/M)(L/I/V/F/M) (SEQ ID NO:

26, P19 consensus), wherein
X at position 1 is optional and can be L, I, V, F, or M;
X at position 3 can be any amino acid, but preferably K, A, S, T, G, D, isoD, E, g-glutamate, Q, N, or R;
X at position 4 can be any amino acid, but preferably A, S, T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
X at position 7 can be any amino acid, but preferably K, A, S, T, G, D, isoD, E, g-glutamate, Q, N, or R;
X at position 10 can be any amino acid, but preferably A, S, T, G, D, isoD, E, g-glutamate, Q, N, K, or R;
and
X at position 11 can be any amino acid, but preferably R, A, S, T, G, D, isoD, E, g-glutamate, Q, N, or K.

5 more elements

An isolated peptide comprising the amino acid sequence of

(i) LXXLLXXLVXLLK (SEQ ID NO:
13, P14d consensus), wherein
X at position 1 can be:
Q, N, D, E, g-glutamate, isoD, or S;
X at position 2 can be:
D, E, g-glutamate, isoD;
X at position 3 can be:
P, D, E, isoD, or g-glutamate;
X at position 4 can be M, A, S, D, E, isoD, or g-glutamate
X at position 5 can be Q, E, or g-glutamate;
X at position 6 can be A, E, or g-glutamate;
X at position 8 can be M, L, E, Q, D, N, G, A, S, isoD, or g-glutamate;
X at position 9 can be Q, N, E, D, G, A, S, isoD, or g-glutamate;
X at position 12 can be Q, N, E, D, G, A, S, isoD, or g-glutamate;
X at position 13 can be Q, N, E, D, G, A, S, isoD, or g-glutamate;
and
X at position 16 can be K, Q, N, E, D, R, G, A, or S;
or
(ii) LXXLLXXLVXLLK (SEQ ID NO:
14, P14d min consensus), wherein
X at position 2 can be M, L, E, Q, D, N, G, A, S, isoD, or g-glutamate;
X at position 3 can be Q, N, E, D, G, A, S, isoD, or g-glutamate;
X at position 6 can be Q, N, E, D, G, A, S, isoD, or g-glutamate;
X at position 7 can be Q, N, E, D, G, A, S, isoD, or g-glutamate;
and
X at position 10 can be K, Q, N, E, D, R, G, A, or S.

23 more elements

An isolated peptide comprising the amino acid sequence of

(i) (L/M)XXLLX(L/M)FXXI(L/M)XX (SEQ ID NO:
15, P3min consensus) wherein
X at position 2 can be Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
X at position 3 can be Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
X at position 6 can be K, Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
X at position 9 can be E, g-glutamate, D, isoD, Q, N, T, S, A, or G;
X at position 10 can be A, G, S, T, E, g-glutamate, D, isoD, Q, or N;
X at position 13 can be Q, N, E, g-glutamate, D, isoD, T, S, A, or G;
and
X at position 14 can be Q, N, E, g-glutamate, D, isoD, T, S, A, or G.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application: 14/872,298Filed 1 Oct 2015
Priority: 1 Oct 2014
Published: 7 Apr 2016
Term ends: Not estimatedThe source records don’t support an estimate.

Classification32 codes

C12N 15/8279
For stress resistance, e.g. heavy metal resistance: for biotic stress resistance, pathogen resistance, disease resistance
A01N 25/02
Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by…
A01N 25/12
Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by…
A01N 37/22
Containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof: the nitrogen atom being directly attached to an…

Family

Application62/058,535Us provisional application · filed 1 Oct 2014
Application62/140,789Us provisional application · filed 31 Mar 2015
US 10,856,546 B2Related patent
US 2017/0156322 A9Published application
US 2016/0095314 A1This patent

From the US filing history. Foreign family members aren’t listed.

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