Recombinase mutants
AssigneeIllumina Cambridge Limited
InventorsErin Bomati, Matthew William Kellinger, Jonathan Mark Boutell
Life of this patent
Estimating the term…
Abstract
Presented herein are recombinases for improved recombinase-mediated amplification of nucleic acids, such as a PCR-library having single-stranded adapter regions, on a patterned flow cell surface for improved cluster amplification, as well as methods and kits using the same.
Claims20
A recombinant UvsX comprising an amino acid sequence that is at least 60%, 70%, 80%, 90%, 95%, 99% identical to SEQ ID NO:
- 1, which recombinant UvsX comprises an amino acid substitution mutation at the position functionally equivalent to Pro256 in the RB49 UvsX amino acid sequence.
A recombinant UvsX comprising the amino acid sequence of any one of SEQ ID NOs:
- 2 and 22-35.
A recombinant UvsX comprising a substitution mutation to the semi-conserved domain comprising the amino acid sequence of any of SEQ ID NOs:
- 3-5 wherein the substitution mutation comprises a mutation selected from a substitution at position 7 to any residue other than Phe, Pro, Asp, Glu or Asn.
A recombinant UvsX comprising a substitution mutation to the semi-conserved domain comprising the amino acid sequence of any of SEQ ID NOs:
- 6-7 wherein the substitution mutation comprises a mutation selected from a substitution at position 12 to any residue other than Phe, Pro, Asp, Glu or Asn.
Dependencies are inferred from the claim text.