Aspartic proteases
AssigneeBiocant-AssociaçãO De TransferêNcia De Tecnologia
InventorsCarla Sofia Gomes Malaquias de Almeida, Isaura Isabel Gonçalves Simões, Carlos José Fialho Costa Faro
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Abstract
The invention relates to aspartic proteases, and particularly to aspartic proteases for plants. Disclosed are modified plant aspartic proteases, and methods for their manufacture, and uses thereof. Particularly contemplated are the uses of aspartic proteases in clotting milk.
Claims28
A plant aspartic protease that is modified so as to lack a functional plant specific insert (PSI) domain.
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12-13. (canceled)
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Nucleic acid encoding:
- i. a plant aspartic protease which lacks a functional PSI domain;
- ii. the polypeptide of SEQ ID NO:
- 1, or SEQ ID NO:
- 2, or
- iii. a polypeptide having at least 70% sequence identity to SEQ ID NO:
- 1, or SEQ ID NO:
- 2, wherein the polypeptide encoded lacks a functional plant specific insert (PSI) domain.
21-23. (canceled)
A method for producing a plant aspartic protease, the method comprising expressing, in a cell that is not a plant cell or plant protoplast, a mutant plant aspartic protease that lacks a functional plant specific insert (PSI) domain.
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Dependencies are inferred from the claim text.