Lone star virus
AssigneesThe Regents of the University of California, The United States of America, as represented by the Secretary, Department of Health and Human
InventorsCharles Chiu, Andrea Swei, Barbara J.B. Johnson
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Abstract
This disclosure concerns the isolation, identification and sequencing of a unique Phlebovirus, the Lone Star Virus. Lone Star Virus nucleic acid molecules, proteins and antibodies are disclosed. Methods are also disclosed for the detection, diagnostic and treatment of the Lone Star Virus.
Claims36
An isolated nucleic acid molecule comprising a nucleic acid sequence at least 90% identical to an open reading frame of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3.
The isolated nucleic acid molecule of claim 6, comprising the nucleic acid sequence set forth as SEQ ID NO:
- 1, SEQ ID NO:
- 2 or SEQ ID NO:
- 3.
A cDNA comprising:
- (a) a nucleotide sequence at least 90% identical to an open reading frame of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- (b) an open reading frame of the nucleic acid sequence set forth as SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- or
- (c) a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence set forth as SEQ ID NOs:
- 4-7.
(canceled)
An isolated oligonucleotide 12 to 40 nucleotides in length, wherein the oligonucleotide specifically hybridizes to one of SEQ ID NO:
- 1, SEQ ID NO:
- 2, or SEQ ID NO:
- 3, or their complement, under high stringency conditions, and wherein the oligonucleotide comprises a fluorophore, a quencher or both.
An isolated monoclonal antibody or antigen-binding fragment thereof that specifically binds to:
- (a) an amino acid sequence encoded by an open reading frame of a nucleic acid sequence at least 90% identical to the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- (b) an amino acid sequence encoded by an open reading frame of a nucleic acid sequence at least 95% identical to the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- or
- (c) an amino acid sequence encoded by an open reading frame of the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:3.
21-22. (canceled)
A kit, comprising
- a container comprising at least one of:
- a) an isolated nucleic acid molecule comprising
- i) a nucleic acid sequence at least 90% identical to an open reading frame of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- ii) an open reading frame of the nucleic acid sequence set forth as SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- (iii) the nucleic acid sequence set forth as SEQ ID NO:
- 1, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- or
- (iv) a cDNA encoding one of SEQ ID NOs:
- 4-7;
- b) a primer that hybridizes to the nucleotide sequence set forth as SEQ ID NO:1, SEQ ID NO:
- 2, SEQ ID NO:
- 3 or their complement under highly stringent conditions,
- c) an isolated polypeptide comprising:
- (i) an amino acid sequence encoded by an open reading frame of a nucleic acid sequence at least 90% identical to the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- (ii) an amino acid sequence encoded by an open reading frame of a nucleic acid sequence at least 95% identical to the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- (iii) an amino acid sequence encoded by an open reading frame of the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- or
- (iv) an immunogenic fragment thereof,
- or d) an antibody that specifically binds to the polypeptide of c);
- and
- e) instructions for using the kit.
27-29. (canceled)
A method of producing an antibody, comprising
- (a) immunizing a mammal with a polypeptide comprising:
- (i) an amino acid sequence encoded by an open reading frame of a nucleic acid sequence at least 90% identical to the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- (ii) an amino acid sequence encoded by an open reading frame of a nucleic acid sequence at least 95% identical to the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- (iii) an amino acid sequence encoded by an open reading frame of the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- or
- (iv) an immunogenic fragment of the polypeptide;
- or a nucleic acid encoding the polypeptide of (i)-(iv);
- and
- (b) isolating an antibody that specifically binds
- (i) the amino acid sequence encoded by an open reading frame of a nucleic acid sequence at least 90% identical to the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- (ii) the amino acid sequence encoded by an open reading frame of a nucleic acid sequence at least 95% identical to the nucleic acid sequence set forth as one SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- or
- (iii) the amino acid sequence encoded by an open reading frame of the nucleic acid sequence set forth as one of SEQ ID NO:
- 1, the complement thereof, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- thereby producing the antibody.
An isolated Phlebovirus comprising an S segment, an M segment and an L segment, wherein:
- (i) the nucleotide sequence of the S segment is at least 80% identical to SEQ ID NO:
- 1;
- (ii) the nucleotide sequence of the M segment is at least 80% identical to SEQ ID NO:
- 2;
- (iii) the nucleotide sequence of the L segment is at least 80% identical to SEQ ID NO:
- 3;
- or
- (iv) all (i), (ii) and (iii).
33-37. (canceled)
A Phlebovirus, wherein the Phlebovirus is attenuated, and wherein the Phlebovirus comprises an S segment, an M segment and an L segment, wherein:
- the nucleotide sequence of the S segment is at least 80% identical to SEQ ID NO:
- 1;
- (ii) the nucleotide sequence of the M segment is at least 80% identical to SEQ ID NO:
- 2;
- (iii) the nucleotide sequence of the L segment is at least 80% identical to SEQ ID NO:
- 3;
- or
- (iv) all (i), (ii) and (iii).
Dependencies are inferred from the claim text.