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US 2016/0079049 A1Published application
Methods for detecting reverse triiodothyronine by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsJ. Fred BANKS, Peter P. CHOU, Noriya M. MATT
Life of this patent
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Abstract
Provided are methods for determining the amount of reverse T3 in a sample using mass spectrometry. The methods generally involve ionizing reverse T3 in a sample and detecting and quantifying the amount of the ion to determine the amount of reverse T3 in the sample.
Claims24
A method for determining the amount of reverse triiodothyronine (rT3) in a sample by tandem mass spectrometry, said method comprising:
- a. adding an internal standard to the sample;
- b. subjecting the sample to protein precipitation;
- c. ionizing rT3 and the internal standard to generate at least one rT3 ion and at least one internal standard ion;
- d. determining the amount of said at least one rT3 ion and the amount of said at least one internal standard ion by tandem mass spectrometry;
- wherein the amount of rT3 in the sample is determined from the amount of said at least one rT3 ion and the amount of said at least one internal standard ion.
2 more elements
A method for determining the amount of reverse triiodothyronine (rT3) in a sample by tandem mass spectrometry, said method comprising:
- a. adding a 13C-labeled rT3 to the sample;
- b. subjecting the sample to protein precipitation;
- c. ionizing rT3 and the 13C-labeled rT3 to generate at least one rT3 ion and at least one 13C-labeled rT3 ion;
- d. determining the amount of said at least one rT3 ion and the amount of said at least one 13C-labeled rT3 ion by tandem mass spectrometry;
- wherein the amount of rT3 in the sample is determined from the amount of said at least one rT3 ion and the amount of said at least one 13C-labeled rT3 ion.
2 more elements
Dependencies are inferred from the claim text.