Engineering microbes and metabolic pathways for the production of ethylene glycol
AssigneeMassachusetts Institute of Technology
InventorsGregory Stephanopoulos, Brian Pereira, Marjan De Mey, Deepak Dugar, Jose Luis Avalos
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Abstract
The invention relates to recombinant cells and their use in the production of ethylene glycol.
Claims31
A cell engineered to produce glycolaldehyde, wherein the cell has reduced or eliminated activity of, or reduced or eliminated expression of, xylulokinase relative to a wild-type cell.
An Escherichia coli cell engineered to produce glycolaldehyde, wherein the cell comprises a deletion of a xylB gene and recombinantly expresses a dte gene obtained from Pseudomonas cichorii, a fucK gene obtained from Escherichia coli, and a fucA gene obtained from Escherichia coli.
An Escherichia coli cell engineered to produce ethylene glycol, wherein the cell comprises a deletion of a xylB gene and an aldA gene, and recombinantly expresses a dte gene obtained from Pseudomonas cichorii, a fucK gene obtained from Escherichia coli, a fucA gene obtained from Escherichia coli, and a fucO gene obtained from Escherichia coli.
23-31. (canceled)
33-39. (canceled)
A cell engineered to produce glycolaldehyde, wherein the cell has reduced or eliminated activity or reduced or eliminated expression of L-ribulokinase relative to a wild-type cell.
41-60. (canceled)
62-64. (canceled)
66-68. (canceled)
Dependencies are inferred from the claim text.