Genes Related to Xylose Fermentation and Methods of Using Same for Enhanced Biofuel Production
AssigneeWisconsin Alumni Research Foundation
InventorsDana J. Wohlbach, Audrey P. Gasch
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Abstract
The present invention provides isolated gene sequences involved in xylose fermentation and related recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast useful for biofuel production are also provided.
Claims18
An isolated nucleic acid having:
- (a) the nucleotide sequence of SEQ ID NO:2 or SEQ ID NO:3;
- or (b) a nucleotide sequence which hybridizes under stringent conditions to SEQ ID NO:2 or SEQ ID NO:3, or to a fully complementary nucleotide sequence thereof, wherein over-expression in yeast of said isolated nucleic acid provides increased xylose fermentation in the yeast relative to a control yeast lacking over-expression of the isolated nucleic acid.
A method for producing ethanol by fermentation of xylose in yeast, comprising:
- (a) culturing under ethanol-producing conditions a recombinant yeast comprising an isolated nucleic acid encoding an aldo/keto reductase (AKR) or a nucleotide sequence which hybridizes under stringent conditions to said isolated nucleic acid encoding AKR, or to a fully complementary nucleotide sequence thereof, and (b) isolating ethanol produced by said recombinant yeast.
A recombinant Saccharomyces cerevisiae strain, comprising (a) an isolated nucleic acid encoding an aldo/keto reductase (AKR) or a nucleotide sequence which hybridizes under stringent conditions to said isolated nucleic acid encoding AKR, or to a fully complementary nucleotide sequence thereof, operably linked to a heterologous promoter;
- and (b) XYL1, XYL2 and XYL3 genes from Pichia stipitis.
Dependencies are inferred from the claim text.