A method and a device for cross-talk correction of measured intensities

A method for cross-talk correction of intensities measured on two or more detection wavelength bands from a sample, each detection wavelength band relating to one of analyte-specific probe-populations contained by the sample and each probe-population being capable of emitting a first signal component and a second signal component whose spectra are mutually different and at least the first signal component being dependent on presence of analyte detectable with that probe-population, the method comprising:

• computing estimates of cross-talk intensities occurring on the detection wavelength bands and at least partially caused by the second signal components emitted by the probe-populations on the basis of:
• a) first cross-talk parameters and b) at least one value indicative of at least one intensity occurring on at least one auxiliary wavelength band outside the detection wavelength bands and at least partially caused by the second signal components, and
• computing cross-talk corrected intensities of the detection wavelength bands on the basis of:
• a) the intensities measured on the detection wavelength bands, b) the computed estimates of the cross-talk intensities, and c) second cross-talk parameters,
• wherein:
• each of the first cross-talk parameters indicates a relation between a) intensity occurring on one of the detection wavelength bands and at least partially caused by the second signal components, and b) intensity occurring on the auxiliary wavelength band and at least partially caused by the second signal components, and
• each of the second cross-talk parameters indicates a relation between a) intensity occurring on the detection wavelength band related to one of the probe-populations and at least partially caused by the first signal component emitted by another of the probe-populations, and b) intensity occurring on the detection wavelength band related to the other of the probe-populations and at least partially caused by the first signal component emitted by the other of the probe-populations.

A device for cross-talk correction of intensities measured on two or more detection wavelength bands from a sample, each detection wavelength band relating to one of analyte-specific probe-populations contained by the sample and each probe-population being capable of emitting a first signal component and a second signal component whose spectra are mutually different and at least the first signal component being dependent on presence of analyte detectable with that probe-population, the device comprising a computing system configured to:

• compute estimates of cross-talk intensities occurring on the detection wavelength bands and at least partially caused by the second signal components emitted by the probe-populations on the basis of:
• a) first cross-talk parameters and b) at least one value indicative of at least one intensity occurring on at least one auxiliary wavelength band outside the detection wavelength bands and at least partially caused by the second signal components, and
• compute cross-talk corrected intensities of the detection wavelength bands on the basis of:
• a) the intensities measured on the detection wavelength bands, b) the computed estimates of the cross-talk intensities, and c) second cross-talk parameters,
• wherein:
• each of the first cross-talk parameters indicates a relation between a) intensity occurring on one of the detection wavelength bands and at least partially caused by the second signal components, and b) intensity occurring on the auxiliary wavelength band and at least partially caused by the second signal components, and
• each of the second cross-talk parameters indicates a relation between a) intensity occurring on the detection wavelength band related to one of the probe-populations and at least partially caused by the first signal component emitted by another of the probe-populations, and b) intensity occurring on the detection wavelength band related to the other of the probe-populations and at least partially caused by the first signal component emitted by the other of the probe-populations.

A measurement instrument comprising:

• measurement equipment (302, 303, 304) for measuring intensities on two or more detection wavelength bands from a sample, each detection wavelength band relating to one of analyte-specific probe-populations contained by the sample and each probe-population being capable of emitting a first signal component and a second signal component whose spectra are mutually different and at least the first signal component being dependent on presence of analyte detectable with that probe-population, and
• a device according for cross-talk correction of the intensities measured on the two or more detection wavelength bands from the sample,
• the device comprising a computing system configured to:
• compute estimates of cross-talk intensities occurring on the detection wavelength bands and at least partially caused by the second signal components emitted by the probe-populations on the basis of:
• a) first cross-talk parameters and b) at least one value indicative of at least one intensity occurring on at least one auxiliary wavelength band outside the detection wavelength bands and at least partially caused by the second signal components, and
• compute cross-talk corrected intensities of the detection wavelength bands on the basis of:
• a) the intensities measured on the detection wavelength bands, b) the computed estimates of the cross-talk intensities, and c) second cross-talk parameters,
• wherein:
• each of the first cross-talk parameters indicates a relation between a) intensity occurring on one of the detection wavelength bands and at least partially caused by the second signal components, and b) intensity occurring on the auxiliary wavelength band and at least partially caused by the second signal components, and
• each of the second cross-talk parameters indicates a relation between a) intensity occurring on the detection wavelength band related to one of the probe-populations and at least partially caused by the first signal component emitted by another of the probe-populations, and b) intensity occurring on the detection wavelength band related to the other of the probe-populations and at least partially caused by the first signal component emitted by the other of the probe-populations.

A non-transitory computer readable medium encoded with a computer program for cross-talk correction of intensities measured on two or more detection wavelength bands from a sample, each detection wavelength band relating to one of analyte-specific probe-populations contained by the sample and each probe-population being capable of emitting a first signal component and a second signal component whose spectra are mutually different and at least the first signal component being dependent on presence of analyte detectable with that probe-population, wherein the computer program comprises computer executable instructions for controlling a programmable processor to:

• compute estimates of cross-talk intensities occurring on the detection wavelength bands and at least partially caused by the second signal components emitted by the probe-populations on the basis of:
• a) first cross-talk parameters and b) at least one value indicative of at least one intensity occurring on at least one auxiliary wavelength band outside the detection wavelength bands and at least partially caused by the second signal components, and
• compute cross-talk corrected intensities of the detection wavelength bands on the basis of:
• a) the intensities measured on the detection wavelength bands, b) the computed estimates of the cross-talk intensities, and c) second cross-talk parameters,
• wherein:
• each of the first cross-talk parameters indicates a relation between a) intensity occurring on one of the detection wavelength bands and at least partially caused by the second signal components, and b) intensity occurring on the auxiliary wavelength band and at least partially caused by the second signal components, and
• each of the second cross-talk parameters indicates a relation between a) intensity occurring on the detection wavelength band related to one of the probe-populations and at least partially caused by the first signal component emitted by another of the probe-populations, and b) intensity occurring on the detection wavelength band related to the other of the probe-populations and at least partially caused by the first signal component emitted by the other of the probe-populations.