Methods and devices for canine herpesvirus 1 (chv-1) detection and prevention
AssigneeThe Board of Regents for Oklahoma State University
InventorSanjay Kapil
Life of this patent
Estimating the term…
Abstract
Methods for detection of canine herpes virus 1 (CHV-1) in archived paraffin-embedded tissue are provided. The methods use CHV-1 glycoprotein B (gB)-specific PCR to detect the CHV-1. A vaginal sleeve that protects puppies from contracting CHV-1 by contact with the mother's vaginal wall during birth is also provided. The sleeve is inserted into the vagina of a pregnant female dog prior to birth and puppies traverse the birth canal through the sleeve without coming into direct contact with vaginal tissue. Compositions and methods of immunizing against CHV-1 in dogs of all ages are also provided.
Claims23
A method of detecting canine herpes virus-1 (CHV-1) in a fixed tissue sample that is embedded in paraffin, comprising
- i) removing paraffin from said fixed tissue sample that is embedded in paraffin;
- ii) isolating DNA from said fixed tissue sample;
- and
- iii) detecting a CHV-1-specific gene in DNA isolated from said fixed tissue sample.
A vaccine or immunogenic composition for eliciting an immune response against CHV-1 in a canine, comprising
- i) an attenuated CHV-1 isolate deposited as ATCC Deposit No. ______, or progeny thereof, or a killed virus corresponding to CHV-1 isolate ______;
- and
- ii) a physiologically compatible carrier.
A method for eliciting an immune response to CHV-1 in a subject in need thereof, comprising administering to said subject a composition comprising
- i) an attenuated CHV-1 deposited as ATCC Deposit No. ______, or progeny thereof, or a killed virus corresponding to CHV-1 isolate ______;
- and
- ii) a physiologically compatible carrier.
A method of preventing vaginal shedding of CVH-1 in a female dog infected with CHV-1, comprising
- contacting vaginal surfaces of said female dog with a composition comprising at least one anti-herpesvirus compound.
A vaginal sleeve to prevent transmission of infectious agents from tissue of a cervix and/or a vagina of a mother to an offspring during birth, comprising
- a substantially cylindrical tube with a distal end and a proximal end, said substantially cylindrical tube possessing an axis with length and a cross sectional area
- wherein
- a length of said axis of said substantially cylindrical tube is equal to or greater than a length of said vagina;
- a cross-sectional area of said tube is sufficient to allow passage of said offspring through said sleeve;
- said distal end comprises an opening bounded by an edge, said edge having a circumference and width sufficient to make direct contact with and cover cervical and/or vaginal tissue adjacent to an opening formed by dilation of said cervix, thereby surrounding said opening;
- and
- said proximal end comprising an opening with a diameter sufficient to allow said offspring to pass through.
Dependencies are inferred from the claim text.