Kanamycin compound, kanamycin-producing streptomyces species bacterium, and method of producing kanamycin
AssigneeIntron Biotechnology, Inc.
InventorsYeo Joon Yoon, Sung Ryeol Park, Je Won Park, Jae Kyung Sohng
Life of this patent
Estimating the term…
Abstract
Vectors expressing kanA-kanB-kanK and other kanamycin production-related genes, Streptomyces species recombinant bacteria transformed with the vectors, a method of producing kanamycin antibiotics by the bacteria, and a new kanamycin compound produced by the bacterium are provided. With the use of the recombinant bacteria of the present invention, the direct fermentative biosynthesis of amikacin and tobramycin as semi-synthetic kanamycins is possible, and the yield of kanamycin B as a precursor of the semi-synthetic kanamycin is improved.
Claims12
A vector comprising at least one gene set selected from the group consisting of:
- (1) kanA, kanB, and kanK;
- (2) kanA, kanB, kanK, and kanF;
- (3) kanA, kanB, kanK, kanF, and kacA;
- (4) kanA, kanB, kanK, kanF, kacA, kanC, and kanD;
- (5) kanA, kanB, kanK, kanF, kacA, kanI, and kacL;
- (6) kanA, kanB, kanK, kanF, kacA, and kanE;
- (7) kanA, kanB, kanK, kanF, kacA, kanE, kanC, and kanD;
- (8) kanA, kanB, kanK, kanF, kacA, kanE, kanI, and kacL;
- (9) kanA, kanB, kanK, kanF, kacA, kanE, kanC, kanD, kanI, and kacL;
- (10) kanA, kanB, kanK, kanF, kacA, kanE, kanC, kanD, btrG, btrH, btrI, btrJ, btrK, btrO, and btrV;
- (11) kanA, kanB, kanK, kanF, kacA, kanE, kanC, kanD, kanI, kacL, btrG, btrH, btrI, btrJ, btrK, btrO, and btrV;
- (12) kanA, kanB, kanK, and nemD;
- (13) kanA, kanB, kanK, nemD, kacA, kanE, kanC, and kanD;
- (14) kanA, kanB, kanK, nemD, kacA, kanE, kanC, kanD, kanI, and kacL;
- (15) kanA, kanB, kanK, nemD, kacA, kanE, kanC, kanD, kanI, kacL, aprD3, and aprD4;
- (16) kanA, kanB, kanK, nemD, kacA, tobM2, kanC, kanD, kanI, kacL, aprD3, and aprD4;
- (17) kanA, kanB, kanK, and tobM1;
- (18) kanA, kanB, kanK, tobM1, kacA, kanE, kanC, and kanD;
- (19) kanA, kanB, kanK, and gtmG;
- and
- (20) kanA, kanB, kanK, gtmG, kacA, kanE, kanC, and kanD.
A Streptomyces species bacterium expressing at least one gene set selected from the group consisting of:
- (1) kanA, kanB, and kanK;
- (2) kanA, kanB, kanK, and kanF;
- (3) kanA, kanB, kanK, kanF, and kacA;
- (4) kanA, kanB, kanK, kanF, kacA, kanC, and kanD;
- (5) kanA, kanB, kanK, kanF, kacA, kanI, and kacL;
- (6) kanA, kanB, kanK, kanF, kacA, and kanE;
- (7) kanA, kanB, kanK, kanF, kacA, kanE, kanC, and kanD;
- (8) kanA, kanB, kanK, kanF, kacA, kanE, kanI, and kacL;
- (9) kanA, kanB, kanK, kanF, kacA, kanE, kanC, kanD, kanI, and kacL;
- (10) kanA, kanB, kanK, kanF, kacA, kanE, kanC, kanD, btrG, btrH, btrI, btrJ, btrK, btrO, and btrV;
- (11) kanA, kanB, kanK, kanF, kacA, kanE, kanC, kanD, kanI, kacL, btrG, btrH, btrI, btrJ, btrK, btrO, and btrV;
- (12) kanA, kanB, kanK, and nemD;
- (13) kanA, kanB, kanK, nemD, kacA, kanE, kanC, and kanD;
- (14) kanA, kanB, kanK, nemD, kacA, kanE, kanC, kanD, kanI, and kacL;
- (15) kanA, kanB, kanK, nemD, kacA, kanE, kanC, kanD, kanI, kacL, aprD3, and aprD4;
- (16) kanA, kanB, kanK, nemD, kacA, tobM2, kanC, kanD, kanI, kacL, aprD3, and aprD4;
- (17) kanA, kanB, kanK, and tobM1;
- (18) kanA, kanB, kanK, tobM1, kacA, kanE, kanC, and kanD;
- (19) kanA, kanB, kanK, and gtmG;
- and
- (20) kanA, kanB, kanK, gtmG, kacA, kanE, kanC, and kanD.
-10. (canceled)
-14. (canceled)
-21. (canceled)
Dependencies are inferred from the claim text.