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US 2016/0032380 A1Published application
Electron beam nucleic acid sequencing
AssigneeCornell University
InventorsHarold G. Craighead, Leon M. Bellan
Life of this patent
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Abstract
The present invention relates to compositions, methods, and uses for obtaining sequence information from nucleic acid molecules.
Claims21
-24. (canceled)
A method for obtaining nucleic acid sequence information, comprising:
- (a) scanning with an electron beam a population of labeled nucleic acid molecules on a substrate, wherein individual nucleic acid molecules of said population of labeled nucleic acid molecules are fixed within a fiber material that is substantially transparent to said electron beam, and wherein said population of labeled nucleic acid molecules is deposited from a tip onto said substrate that is moving in a direction such that said labeled nucleic acid molecules within said population are elongated on said substrate and aligned along said direction of movement of said tip;
- (b) detecting bases of said individual nucleic acid molecules of said population of labeled nucleic acid molecules while scanning said population of labeled nucleic acid molecules with said electron beam;
- and
- (c) generating sequence information of said individual nucleic acid molecules detected in (b), wherein said sequence information includes an identity and order of bases within each of said individual nucleic acid molecules.
1 more element
A method for obtaining nucleic acid sequence information, comprising:
- (a) providing a substrate having a nucleic acid molecule deposited on a surface thereof, wherein said nucleic acid molecule is elongated on said surface, wherein said nucleic acid molecule is fixed within a fiber material that is substantially transparent to an electron beam, and wherein one or more types of bases of said nucleic acid molecule include a detectable label;
- (b) scanning said nucleic acid molecule with said electron beam;
- (c) while scanning said nucleic acid molecule with said electron beam, resolving signals from said surface via electron energy loss spectroscopy (EELS), wherein said signals are indicative of an identity and order of said bases within said nucleic acid molecule;
- and
- (d) using said signals resolved in (c) to obtain sequence information of said nucleic acid molecule, wherein said sequence information includes said identity and order of said bases within said nucleic acid molecule.
2 more elements
Dependencies are inferred from the claim text.