Methods for detecting peptide/mhc/tcr binding

A method for detecting binding of a peptide to an MHC molecule, comprising:

• contacting said MHC molecule with a polynucleotide-peptide conjugate, said polynucleotide-peptide conjugate comprising said peptide and a polynucleotide;
• contacting said polynucleotide-peptide conjugate with a probe that specifically binds to said polynucleotide;
• detecting binding of said probe to said polynucleotide;
• and
• correlating binding of said probe to said polynucleotide with binding of said peptide to said MHC molecule.

A method for simultaneously detecting binding of a library of peptides to an MHC molecule, comprising:

• providing a polynucleotide-peptide conjugate for each said peptide, said polynucleotide-peptide conjugate comprising said peptide and a polynucleotide;
• contacting said MHC molecule with a pool of said polynucleotide-peptide conjugates;
• contacting each of said polynucleotide-peptide conjugate with a probe that specifically binds to each said polynucleotide;
• detecting binding of said probe to each corresponding polynucleotide that each said probe specifically binds;
• and
• correlating binding of said probe to each corresponding polynucleotide with binding of each corresponding peptide to said MHC molecule.

A method for detecting in a library of peptides competitive binding of each said peptide to an MHC molecule, comprising:

• providing a polynucleotide-peptide conjugate for each said peptide, said polynucleotide-peptide conjugate comprising said peptide and a polynucleotide;
• contacting said MHC molecule with a pool of said polynucleotide-peptide conjugates;
• contacting each of said polynucleotide-peptide conjugate with a probe that specifically binds to each said polynucleotide;
• detecting binding of said probe to each corresponding polynucleotide that each said probe specifically binds;
• and
• correlating binding of said probe to each corresponding polynucleotide with binding of each corresponding peptide to said MHC molecule,
• wherein said peptides compete for binding of said MHC molecule.

A method of detecting binding of a peptide to a TCR, comprising:

• contacting said TCR with an MHC molecule and a polynucleotide-peptide conjugate, said polynucleotide-peptide conjugate comprising said peptide and a polynucleotide;
• contacting said polynucleotide-peptide conjugate with a probe that specifically binds to said polynucleotide;
• detecting binding of said probe to said polynucleotide;
• and
• correlating binding of said probe to said polynucleotide with binding of said peptide to said TCR.

A method for simultaneously detecting binding of a library of peptides to a TCR, comprising:

• providing a polynucleotide-peptide conjugate for each said peptide, said polynucleotide-peptide conjugate comprising said peptide and a polynucleotide;
• contacting said TCR with a pool of said polynucleotide-peptide conjugates and an MHC molecule;
• contacting each of said polynucleotide-peptide conjugate with a probe that specifically binds to each said polynucleotide;
• detecting binding of said probe to each corresponding polynucleotide that each said probe specifically binds;
• and
• correlating binding of said probe to each corresponding polynucleotide with binding of each corresponding peptide to said TCR.

A method for detecting in a library of peptides competitive binding of each said peptide to a TCR, comprising:

• providing a polynucleotide-peptide conjugate for each said peptide, said polynucleotide-peptide conjugate comprising said peptide and a polynucleotide;
• contacting said TCR with a pool of said polynucleotide-peptide conjugates and an MHC molecule;
• contacting each of said polynucleotide-peptide conjugate with a probe that specifically binds to each said polynucleotide;
• detecting binding of said probe to each corresponding polynucleotide that each said probe specifically binds;
• and
• correlating binding of said probe to each corresponding polynucleotide with binding of each corresponding peptide to said TCR,
• wherein said peptides compete for binding of said MHC molecule and said TCR.