Fragment complementation of based assays

A method comprising:

• a) contacting a sample comprising one or more target nucleic acids with one or more capture oligonucleotide probes immobilized on a surface under conditions that permit the target nucleic acids to hybridize with the capture oligonucleotide probes, thereby forming one or more captured target nucleic acids;
• b) contacting one or more detection elements each comprising a target interacting probe associated with an a-fragment peptide, so that the detection elements interact with the captured target nucleic acids under conditions that permit the captured target nucleic acids to hybridize with the target interaction probes, thereby forming one or more oligocomplexes;
• c) contacting the oligocomplexes with one or more inactive mutant β-galactosidases under conditions that permit complementation of the α-fragment peptides of the oligocomplexes with the inactive mutant β-galactosidases, thereby generating one or more enzymatically active entities each comprising an activated β-galactosidase unit;
• and
• d) determining presence and/or amount of the target nucleic acids by detecting enzyme activity of the activated β-galactosidase unit.

A kit comprising:

• a) one or more copies of at least one capture probe immobilized on a surface, wherein the capture probe is characterized by an ability to associate with an analyte in a sample under predetermined conditions, thereby forming at least one captured analyte;
• b) at least one detection element, comprising a target interacting probe associated with a first subunit of a detectable entity, wherein the target interacting probe is characterized by an ability to associate with the captured analyte under predetermined conditions so that at least one first complex, comprising the capture probe, the analyte, the target interacting probe, and the first subunit is formed;
• and
• c) at least one second subunit being characterized by an ability to, when associated with the first subunit, complements the first subunit and generates a detectable entity, under predetermined conditions, wherein the presence and/or amount of the analyte is indicated by detecting level or activity of the detectable entity.

A kit comprising:

• a) a population of encoded surfaces comprising one or more sub-populations, wherein the sub-populations differing from one another in that:
• i) each sub-population has a signature distinguishable from one another;
• and
• ii) each sub-population carriers one or more copies of a single capture probe, wherein the capture probe is characterized by an ability to associate with a particular one of one or more analytes in a sample under predetermined conditions, thereby forming a particular captured analyte;
• b) one or more detection elements, each comprising a target interacting probe associated with a first subunit of a detectable entity, wherein the target interacting probe is characterized by an ability to associate with the particular captured analyte under predetermined conditions so that the particular first complex, comprising the capture probe, the particular analyte, the target interacting probe, and the particular first subunit is formed;
• and
• c) one or more second subunits each being characterized by an ability to, when associated with the first subunit, complements the first subunit and generates a particular detectable entity, under predetermined conditions, wherein the presence and/or amount of the particular analyte is indicated by detecting level or activity of the particular detectable entity.