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AssigneeTheranos, Inc.
InventorsDouglas Matje, Ian Gibbons, Paul Patel, Elizabeth A. Holmes
Estimating the term…
Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures.
1-20. (canceled)
A reagent for use in a cholesterol assay, said reagent comprising a lipoprotein solubilization agent, lipoprotein interactant, and a buffer, wherein said lipoprotein interactant is present at a concentration level that provides for conversion of high density lipoprotein cholesterol (HDL-C) to a measureable colored product at a substantially different rate than for conversion of low density lipoprotein cholesterol (LDL-C) to a measureable colored product and at a substantially different rate than for conversion of very low density lipoprotein cholesterol (VLDL-C) to a measureable colored product when said reagent is used in a cholesterol assay.
A reagent for use in an assay for the simultaneous, rapid measurement of at least two of total cholesterol, low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) in lipoproteins in a sample of blood from a subject without substantial precipitation of said lipoproteins, comprising a buffer, an amphiphilic agent, a lipase comprising a cholesterol esterase, an oxidase comprising a cholesterol oxidase, and a colorant.
A kit comprising a reagent and instructions for the use of said reagent in a cholesterol assay, the reagent comprising α-cyclodextrin sulfate, dextran sulfate, magnesium chloride, 4-aminoantipyrene and a sodium phosphate buffer.
A kit comprising a reagent and instructions for the use of said reagent in a cholesterol assay, the reagent comprising a sodium phosphate buffer, Triton X-100, pluronic L64, N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline (ALPS), cholesterol esterase from Pseudomonas sp., and cholesterol oxidase from Pseudomonas sp.
Dependencies are inferred from the claim text.