Method for preparing d-chiro-inositol using microbes
AssigneeDy Natural Co., Ltd.,
InventorsHyun Koon MYEONG, Sang-Hwal YOON, Hyeon-Seo LEE
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Abstract
The present invention relates to a method for preparing D-chiro-inositol from myo-inositol by using a transformed host cell which expresses enzymes such as a myo-inositol transporter, inositol dehydrogenase, and inosose isomerase. According to the method of the present invention, myo-inositol can be converted into D-chiro-inositol at a high yield.
Claims17
A method for preparing D-chiro-inositol from myo-inositol, the method comprising:
- (a) obtaining a transformed host cell by transforming a host cell with (i) a recombinant vector including a myo-inositol transporter coding DNA sequence operatively linked to a promoter;
- (ii) a recombinant vector including an inositol dehydrogenase coding DNA sequence and an inosose isomerase coding DNA sequence operatively linked to a promoter;
- and
- (b) culturing the transformed host cell in a medium including myo-inositol.
A host cell transformed with a recombinant vector, the recombinant vector including:
- (i) a myo-inositol transporter coding DNA sequence operatively linked to a promoter;
- and (ii) an inositol dehydrogenase coding DNA sequence and an inosose isomerase coding DNA sequence which are operatively linked to a promoter.
A recombinant vector kit for preparing D-chiro-inositol from myo-inositol, comprising:
- (i) a recombinant vector including a myo-inositol transporter DNA coding sequence operatively linked to a promoter;
- and (ii) a recombinant vector including an inositol dehydrogenase coding DNA sequence and an inosose isomerase coding DNA sequence operatively linked to a promoter as an active component.
Dependencies are inferred from the claim text.