Compositions and methods of use of acc oxidase polynucleotides and polypeptides
AssigneePioneer Hi-Bred International, Inc.
InventorsXIAOMING BAO, JEFFREY HABBEN, SABRINA HUMBERT
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Abstract
Transgenic compositions and methods reduce the expression of endogenous ACC oxidase genes to improve an agronomic characteristic of a crop plant, which may be maize. Yield increase and drought tolerance due to reduction in the endogenous ACC oxidase levels are observed. ACC oxidase genes are identified in maize, rice, and Arabidopsis genomes.
Claims30
A method of improving abiotic stress tolerance in a crop plant, the method comprising reducing the expression of an ACC oxidase (ACO) gene in the crop plant and growing the crop plant in a plant growing environment, wherein the crop plant is exposed to an abiotic stress, and wherein the ACC oxidase gene that is down regulated comprises a polynucleotide encoding a polypeptide at least 95% identical to the full length of a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 21-30, 59, 61, 63, 65, 67, 69, 71 and 81-91.
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A transgenic maize plant comprising in its genome a recombinant nucleic acid that down regulates the expression of an endogenous ACO gene, wherein the ACO gene comprises a polynucleotide that encodes a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 21-30 and 81-91.
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A method of down-regulation of an endogenous ACC oxidase gene in a maize plant, the method comprising expressing a recombinant nucleic acid construct that reduces the expression of the endogenous ACC oxidase selected from the group consisting of SEQ ID NOS:
- 1-20 or an allelic variant of the sequences thereof.
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Dependencies are inferred from the claim text.